Histone H3.3 antibody LS-C383945 is an unconjugated rabbit polyclonal antibody to Histone H3.3 (10-90 aa) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB.RabbitH3 histone, family 3AHuman,Mouse ,RatUnconjugatedELISA,WB

Western blot of Histone H3.3 antibody of LSM bio (10?90 aa, IHC, IF, WB) LS?C383945

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.雙抗體夾心法測定標(biāo)本中組蛋白H3.3水平。用純化的組蛋白H3.3抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入組蛋白H3.3，再與HRP標(biāo)記的組蛋白H3.3抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的組蛋白H3.3呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計算樣品中組蛋白H3.3濃度。
組蛋白H3.3抗體是一類可以特異性結(jié)合組蛋白H3.3的多克隆抗體，主要用于檢測組蛋白H3.3的Western Blot、IHC-P、IF、ELISA、Co-IP等多種免疫學(xué)實驗。