Identification | More | [Name]
4-Aminophthalhydrazide | [CAS]
3682-14-2 | [Synonyms]
4-AMINOPHTHALHYDRAZIDE 4-AMINOPHTHALOYLHYDRAZINE 6-AMINO-2,3-DIHYDRO-1,4-PHTHALAZINEDIONE 6-AMINO-2,3-DIHYDROPHTHALAZINE-1,4-DIONE ISOLUMINOL 4-AMINOPHTHALHYDRAZIDE HYDRATE, 98% 4-AMINOPHTHALHYDRAZIDE MONOHYDRATE 6-AMINO-2-3-DIHYDRO-1-4-PHTHALAZINEDIONE CRYSTALLI 6-Amino-2,3-dihydro-1,4-phthal- 4-AMINOPHTHALHYDRAZIDE MONOHYDRATE BIOCHEMIKA, FOR CHEMILUMINESCENCE 99% Isoluminol [Chemiluminescence reagent] 6-Amino-2,3-dihydro-1,4-phthalazinedione, Isoluminol 6-Amino-1,2,3,4-tetrahydrophthalazine-1,4-dione | [Molecular Formula]
C8H7N3O2 | [MDL Number]
MFCD00010560 | [Molecular Weight]
177.16 | [MOL File]
3682-14-2.mol |
Chemical Properties | Back Directory | [Melting point ]
300 °C (lit.) | [density ]
1.433±0.06 g/cm3(Predicted) | [storage temp. ]
Keep in dark place,Inert atmosphere,Room temperature | [solubility ]
Soluble in ammonium hydroxide, sodium carbonate or other base. | [form ]
Solid | [pka]
10.78±0.20(Predicted) | [color ]
White to Off-White | [Sensitive ]
Air Sensitive | [BRN ]
164804 | [InChI]
InChI=1S/C8H7N3O2/c9-4-1-2-5-6(3-4)8(13)11-10-7(5)12/h1-3H,9H2,(H,10,12)(H,11,13) | [InChIKey]
HUDPLKWXRLNSPC-UHFFFAOYSA-N | [SMILES]
C1(=O)C2=C(C=CC(N)=C2)C(O)=NN1 | [CAS DataBase Reference]
3682-14-2(CAS DataBase Reference) |
Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . S36:Wear suitable protective clothing . | [WGK Germany ]
3
| [F ]
10-23 | [HS Code ]
29280000 |
Hazard Information | Back Directory | [Chemical Properties]
Powder | [Biochem/physiol Actions]
4-Aminophthalhydrazide or isoluminol is a chemiluminescent compound that comprises a phthalazine ring. It is more hydrophilic than luminol. Isoluminol is commercially used in a wide variety of in vitro immunoassays and is a chemiluminescence amplifier for studying reactive oxygen species. It is also useful as an electroluminescence (ECL) biosensor and as a probe for rapid microbial detection. | [Synthesis]
The general procedure for the synthesis of 4-amino phthalazine hydrazide using 6-nitro-2,3-dihydrophthalazine-1,4-dione as starting material was as follows: Intermediate B was transferred to a 5L high-pressure hydrogenation reactor and 3 g of 10% palladium/carbon catalyst was added. The reaction system was heated to 35 ℃ and hydrogen was introduced until the pressure reached 4 atm, and the reaction was maintained at a constant pressure for 4 h. After the reaction was completed, the reaction was carried out with nitrogen. Upon completion of the reaction, the hydrogen in the reactor was replaced with nitrogen. Subsequently, the pH of the reaction solution was adjusted to 3-4 with 35% by weight concentrated hydrochloric acid. heating was continued to 100°C and the nitrogen gas flow was stopped. The reaction mixture was filtered while hot and the filtrate precipitated a large amount of solid after cooling to room temperature. The solid precipitate was collected by filtration and the filtrate could be used for organic solvent recovery. The resulting filter cake was washed with 400 ml of deionized water and dried to give 214 g of 4-amino phthalic hydrazide (isluminolide). | [References]
[1] Patent: CN106810501, 2017, A. Location in patent: Paragraph 0050 [2] Journal of the Chemical Society, 1937, p. 26,31 [3] Recueil des Travaux Chimiques des Pays-Bas, 1935, vol. 54, p. 471 [4] Bulet.Soc.Chim.Romania, 1930, vol. 12, p. 140,163 [5] Patent: CN105348202, 2016, A. Location in patent: Paragraph 0045; 0054; 0055; 0056 |
|
|