N-乙?；窠?jīng)鞘氨醇
| 中文名稱 | N-乙?；窠?jīng)鞘氨醇 |
|---|---|
| 中文同義詞 | N-乙?；窠?jīng)鞘氨醇;N-乙?；?D-神經(jīng)鞘氨醇;C2 神經(jīng)酰胺;N-[(1S,2R,3E)-2-羥基-1-(羥甲基)-3-庚烯-1-基]-乙酰胺;N-乙酰鞘氨醇;化合物 T22644;N-乙?；?D-鞘氨醇;化合物 C2 CERAMIDE |
| 英文名稱 | C2 CERAMIDE |
| 英文同義詞 | N-ACETYL-D-ERYTHRO-SPHINGOSINE;N-ACETYLSPHINGOSINE, D-ERYTHRO;C14 SPHINGOID BASE;C2:0 CERAMIDE;C2-D-ERYTHRO-CERAMIDE;C2 CERAMIDE;C2 CERAMIDE OF D-ERYTHRO-C14-SPHINGOSINE;CERAMIDE C2 |
| CAS號 | 3102-57-6 |
| 分子式 | C20H39NO3 |
| 分子量 | 341.53 |
| EINECS號 | |
| 相關(guān)類別 | 生化試劑;小分子;Mixed Fatty Acids;Activators;Fatty Acid Derivatives & Lipids;Glycerols;Inhibitors;Protein Kinase Inhibitors and Activators;Lipid signaling |
| Mol文件 | 3102-57-6.mol |
| 結(jié)構(gòu)式 |  |
N-乙酰基神經(jīng)鞘氨醇 性質(zhì)
| 熔點(diǎn) | 93-96 °C |
|---|---|
| 沸點(diǎn) | 477.13°C (rough estimate) |
| 密度 | 1.0283 (rough estimate) |
| 折射率 | 1.5614 (estimate) |
| 儲存條件 | −20°C |
| 溶解度 | 可溶于DMSO |
| 酸度系數(shù)(pKa) | 13.50±0.20(Predicted) |
| 形態(tài) | 粉末 |
| 顏色 | 白色 |
| 生物來源 | semisynthetic |
| 穩(wěn)定性 | 濕氣敏感 |
Protein phosphatase 1
PP2A
Ceramide-activated protein phosphatase (CAPP)
Apoptosis
Mitochondrial respiratory chain complex III
C2 Ceramide (5 nM-200 μM; 24 hours; primary mouse osteoblasts) treatment (≤500 nM) promots osteoblast viability, whilst concentrations ≥2 μM significantly reduces osteoblast viability in a dose- and time-dependent manner.
C2 Ceramide increases cytoplasmic histone-associated DNA fragments by 5.7- and 11.2-fold at 50 μM and 100 μM C2 Ceramide concentrations respectively in osteoblasts. At these higher concentrations, C2 Ceramide is a potent inducer of apoptosis in osteoblasts.
C2 Ceramide up-regulates mRNA expression of angiogenic genes in human dental pulp cells (HDPCs) and increases the migration and capillary tube formation of endothelial cells, whereas PP1 small interfering RNA shows opposite effects. Human dental pulp cells (HDPCs) increases levels of bone morphogenetic protein 2, phosphorylation of Smad 1/5/8, and mRNA expression of runt-related transcription factor 2 and osterix.
Cell Viability Assay
| Cell Line: | Primary mouse osteoblasts |
| Concentration: | 5 nM-200 μM |
| Incubation Time: | 24 hours |
| Result: | Murine osteoblasts demonstrated a dose-dependent increase in their survival rate when exposed to low concentrations of 5-500 n?M. Increasing concentrations of 20-200μM caused a dose-dependent decrease in mitochondrial succinate dehydrogenase activity and osteoblast survival. |
The PP1 activator C2 Ceramide increases alkaline phosphatase activity, mineralizes nodule formation, and mRNA expression of dentin matrix protein 1 and dentin sialophosphoprotein. In contrast, knockdown by PP1 small interfering RNA inhibits odontoblastic differentiation.
安全信息
| 安全說明 | 22-24/25 |
|---|---|
| WGK Germany | 3 |
| 海關(guān)編碼 | 29241990 |
| 提供商 | 語言 |
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