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Analytical Chemistry

Analytical chemistry is the subject for the method and basic principle of studying and identifying of the composition, status, structure of matter as well as determination of related content. It is an important branch of chemistry subject. Analytical chemistry plays an important role in not only its own development but also in various fields related to the chemistry. We can say that all the practice of any human activity involving chemical phenomena is inseparable from analytical chemistry. Now, people have developed various kinds of different analytical methods, which can be classified based on an analysis task, analysis object, the basis of the analysis, requirement of the analysis and sample dosage.

According to the analysis tasks, it can be divided into qualitative analysis, quantitative analysis and structural analysis. Task of qualitative analysis is to identify the elements, radicals, functional groups or compounds that constituting the substances; the task of the quantitative analysis is to determine the content of the related components in the sample; the task of structural analysis is to study the molecular structure or crystal structure of the material.

(1) According to the analysis objects, it can be divided into organic and inorganic analysis; the object for the inorganic analysis is inorganic substance; the object of organic analysis is organic substance. In the inorganic analysis, it is generally required to determine what elements, ions, radicals or compounds that constitutes the sample and measure the percentage of each component; and sometimes it is also necessary for determination of the crystal structure; in the organic analysis, it not only requires the identification of the constituent elements, but also, more importantly, should do the structure analysis and functional group analysis.
(2) According to whether the analysis is based on the physical properties or chemical properties of the substance, it can be divided into instrumental analysis and chemical analysis. Depending on the specific requirements, it can be divided into routine analysis, rapid analysis and arbitration Analysis. Routine analysis refers to the general daily laboratory production analysis, also known as conventional analysis; rapid analysis is a kind of routine analysis and is mainly applied to the control of the production process, demanding the report of the results in the shortest possible time with the error generally being allowed to be greater; arbitration analysis is needed when there is controversy in the analysis results from different institute, demanding related department to conduct accurate analysis using specific method in order to determine the accuracy of the results of the original analysis.
(3) According to the amount of the sample, it can be generally divided into constant (> 0.1g), semi-micro (0.01 ~ 0.1g) and trace (1 ~ 10mg) analysis.
(4) In the inorganic qualitative chemical analysis, people generally apply semi-micro method while people generally apply constant analysis in the quantitative chemical analysis. According to the relative amounts of the analyzed components contained in the sample, it is also roughly divided into constant component analysis (> 1%), minor component analysis (0.01 to 1%) and trace components analysis (<0.01%). For the analysis of some trace amount of components contained in some kinds of complicated mixture and some substances, it is necessary to perform separation and enrichment. This produces a series of separation techniques, such as extraction, distillation, ion exchange, chromatography, sedimentation and flotation separation, these chemical separation techniques are an integral part of the analysis.

Environmental Analytical Chemistry
Environmental Analytical Chemistry is briefly referred to environmental analysis. It is a kind of subject to study the types, components of pollutants in the environment as well as how to perform qualitative and quantitative analysis on the chemical contaminants in the environment. It is a branch of environmental chemistry.

Environmental analytical chemistry emerged, developed and improved during the process of solving environmental problems. In 1950s, the public nuisance disease occurred in Japan had alerted the whole world. In order to find the cause of public nuisance disease, after experiencing as long as 11 years, later, the chemists of environmental analysis had applied light spectrum and identified that the river in Itai-itai disease area contained harmful elements such as lead, cadmium, arsenic and so on. Further by means of tracking element analysis of the soil and food in the disease area, people had found high lead and cadmium content. Later, people had further conducted spectral quantitative analysis on the body and bone of the patients in the disease area and found that the bone ashes contained alarmingly high content of zinc, lead and cadmium. To determine the causative agent, people further incorporated zinc, lead and cadmium into the food for feeding animals and conduct trace elemental analysis for animals and confirmed the serious harm of cadmium on the bone, revealing the cause of the Itai-itai disease. The development of modern science, especially the development of modern chemistry, physics, mathematics, electronics, biology, as well as the emergence of accurate, reliable, sensitive, selective, rapid, simple environmental pollution analysis technology and automation equipment, has been resulting in the maturation of environmental analytical chemistry. Environmental analytical chemistry now has penetrated into all areas of the entire environmental science subject. It is the most effective means of access to environmental information quality.
The objects of the environmental analytical chemistry research are quite complicated, including air, water, soil, sediment, minerals, waste, animals, plants, food, and human tissue. The content of chemical elements or compound to be determined in the environmental analytic chemistry is very low, with the absolute content being within the level of 10-6 to 10-12 grams.


The analysis technology in the environmental analytical chemistry is developing towards the direction of continuous automation, computerization and joint combination of various methods and instruments. Currently applied automatic analysis methods include colorimetric analysis, ion selective electrode, x-ray fluorescence spectroscopy, atomic absorption spectroscopy, polarography, gas chromatography, liquid chromatography and flow injection analysis. Laser, as the light source of analytical chemistry technique, has also been applied. Since the laser analysis has properties of high resolution, high sensitivity, long-range and short-term, the laser technology will play a pivotal role in the development of environmental analytical chemistry.

With the deepened development of environmental science, environmental analytical chemistry is often demanded for trace levels and ultra-trace-level detection and analysis, therefore, high sensitivity. Thus study of analysis methods of high sensitivity, good selectivity, rapid trace and ultra trace will become the major development direction for environmental analysis in the near future.

Qualitative Analysis of Chemistry
Qualitative analytic chemistry is the subject to identify the chemical elements and atoms groups contained in the sample. It is a branch subject of the analysis chemistry. Its purpose is to ascertain the chemical composition of the research object (specimen).
The major research content of the qualitative analytic chemistry includes:

1 the tested samples were analyzed separately. Namely take part of the sample and use exclusive reaction to detect a desire detection component.
2 systematic analysis of the samples. This means successively apply a few selective reactions for gradual separation of the ions followed by separation of each group until separating to only one substance and finally apply confirming reaction to ascertain the existence of this substance. The most famous cation system analysis method is H2S system. In recent years, due to the use of advanced equipment, qualitative analysis has also rapidly developed together with multivariate analysis and has also become an important direction for analytical chemistry.

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Structure Chemical Name CAS MF
Avanafil Impurity 10 Avanafil Impurity 10
Capecitabine Impurity 10 Capecitabine Impurity 10
Carfilzomib Impurity C Carfilzomib Impurity C
Cefathiamidine Impurity 4 Cefathiamidine Impurity 4 1418224-75-5 C10H11BrN2O5S
Cefodizime Impurity 2 Cefodizime Impurity 2
Choline Fenofibrate Impurity 1 Choline Fenofibrate Impurity 1 2985-79-7 C13H9ClO2
DecitaBine Impurity 11 DecitaBine Impurity 11
Isavuconazole Impurity 21 Isavuconazole Impurity 21
Isavuconazole Impurity 27 Isavuconazole Impurity 27
Istradefylline Impurity E Istradefylline Impurity E
Linagliptin Impurity 41 Linagliptin Impurity 41
PalBociclib Impurity 17 PalBociclib Impurity 17
PalBociclib Impurity 24 PalBociclib Impurity 24
Posaconazole Impurity 12 Posaconazole Impurity 12
Posaconazole Impurity 24 Posaconazole Impurity 24 165115-78-6 C19H20F2O2
Sitagliptin Impurity 24 Sitagliptin Impurity 24 2349567-54-8 C15H19F2NO4
Tamoxifen EP Impurity H Tamoxifen EP Impurity H 809285-09-4 C43H48N2O2
Tenofovir impurity Q Tenofovir impurity Q
(Z)-2-(2-Aminothiazol-4-yl)-2-(1-carboxy-1-methyl)ethoxyiminoacetic acid (Z)-2-(2-Aminothiazol-4-yl)-2-(1-carboxy-1-methyl)ethoxyiminoacetic acid 102507-85-7 C9H11N3O5S
Cefaclor EP IMpurity Cefaclor EP IMpurity
Alogliptin Impurity B Alogliptin Impurity B 865758-98-1 C17H19N5O2
Alogliptin Impurity B Alogliptin Impurity B 865758-98-1 C17H19N5O2
Linagliptin Impurity F Linagliptin Impurity F 1673546-62-7 C27H27F3N8O3
Parecoxib Impurity D Parecoxib Impurity D
Parecoxib Impurity I Parecoxib Impurity I
1-Hydroxymethyl-beta-carboline glucoside 1-Hydroxymethyl-beta-carboline glucoside 1408311-12-5 C18H20N2O6
Axitinib Impurity D Axitinib Impurity D 1443118-72-6 C29H25N5OS
Lenvatinib Impurity d Lenvatinib Impurity d 417717-04-5 C20H17ClN4O4
4-bromo-3-(hydroxymethyl)phenol 4-bromo-3-(hydroxymethyl)phenol 2737-20-4 C7H7BrO2
4-(4-Bromo-3-formyl-phenoxy)-benzonitrile 4-(4-Bromo-3-formyl-phenoxy)-benzonitrile 906673-54-9 C14H8BrNO2
Atracurium Impurity E Atracurium Impurity E
Trelagliptin Impurity 20 Trelagliptin Impurity 20 1821520-42-6 C20H22FN5O3
Umeclidinium Impurity 3 Umeclidinium Impurity 3 869113-45-1 C29H32BrNO3
Vardenafil Impurity 7 Vardenafil Impurity 7 448184-56-3 C21H26N6O7S
Moxifloxacin Related Compound A (HCl salt form) Moxifloxacin Related Compound A (HCl salt form) 151282-23-4 C20H22ClF2N3O3
8-Hydroxy Entecavir 8-Hydroxy Entecavir 1795791-10-4 C12H15N5O4
1-Pyrrolidinecarboxylic acid, 4-[4-(3-methyl-1-phenyl-1H-pyrazol-5-yl)-1-piperazinyl]-2-(3-thiazolidinylcarbonyl)-, 1,1-dimethylethyl ester, (2S,4R)- 1-Pyrrolidinecarboxylic acid, 4-[4-(3-methyl-1-phenyl-1H-pyrazol-5-yl)-1-piperazinyl]-2-(3-thiazolidinylcarbonyl)-, 1,1-dimethylethyl ester, (2S,4R)- 1404559-22-3 C27H38N6O3S
Ampicillin Impurity A Ampicillin Impurity A
Cefditoren Pivoxil Dimer Cefditoren Pivoxil Dimer
Cefixime Impurity A(EP) Cefixime Impurity A(EP)
Oxacillin Sodium Impurity  B(EP) Oxacillin Sodium Impurity B(EP)
3,5-dichlorothiophene-2-carboxylic acid 3,5-dichlorothiophene-2-carboxylic acid 89166-94-9 C5H2Cl2O2S
Brexpiprazole Impurity 2 Brexpiprazole Impurity 2 2094559-58-5 C38H40N4O4S
2-methyl-6-oxo-1,6-dihydro-[3,4'-bipyridine]-5-carboxylic acid 2-methyl-6-oxo-1,6-dihydro-[3,4'-bipyridine]-5-carboxylic acid 80047-38-7 C12H10N2O3
Ninhydrin hydrate Ninhydrin hydrate 485-47-2 C9H6O4
Levofloxacin Impurity 7 Levofloxacin Impurity 7 1607796-83-7 C15H14FNO5
Moxifloxacin EP Impurity E HCl Moxifloxacin EP Impurity E HCl
Pantoprazole Impurity 11 Pantoprazole Impurity 11 172282-50-7 C7H8F2N2O
Solifenacin Related Compound 24 Solifenacin Related Compound 24 1682642-69-8 C15H23ClN2
Ticagrelor Related Compound 44 Ticagrelor Related Compound 44 1431867-40-1 C8H12ClN3OS
(trans)-2-(3,4-difluorophenyl)cyclopropanecarboxamide (trans)-2-(3,4-difluorophenyl)cyclopropanecarboxamide 1006614-51-2 C10H9F2NO
2-cyano-N-methyl-N-((3R,4R)-4-methyl piperidin-3-yl) Acetamide hydrochloride 2-cyano-N-methyl-N-((3R,4R)-4-methyl piperidin-3-yl) Acetamide hydrochloride 1640971-87-4 C10H18ClN3O
6-methyl-N1-(4-methyl-6-(pyridin-3-yl)pyrimidin-2-yl)benzene-1,3-diamine 6-methyl-N1-(4-methyl-6-(pyridin-3-yl)pyrimidin-2-yl)benzene-1,3-diamine C17H17N5
5-METHOXY-2-BENZIMIDAZOLINONE 5-METHOXY-2-BENZIMIDAZOLINONE 2080-75-3 C8H8N2O2
(2R,4S)-4-([1,1'-Biphenyl]-4-ylmethyl)-2-methyl-4-(2,5-dioxopyrrolidin-1-yl)butanoic acid (2R,4S)-4-([1,1'-Biphenyl]-4-ylmethyl)-2-methyl-4-(2,5-dioxopyrrolidin-1-yl)butanoic acid 1639970-62-9 C22H23NO4
Linagliptin Impurity I Linagliptin Impurity I
1H-Pyrrole-3-carbonitrile,5-(2-fluorophenyl)-1-(3-pyridinylsulfonyl)- 1H-Pyrrole-3-carbonitrile,5-(2-fluorophenyl)-1-(3-pyridinylsulfonyl)- 1807642-39-2 C16H10FN3O2S
4-amino-1-[(2RS)-2-(2,4-difluorophenyl)-2-hydroxy-3(1H-
1,2,4-triazol-1-yl)propyl]-4H-1,2,4-triazolium 4-amino-1-[(2RS)-2-(2,4-difluorophenyl)-2-hydroxy-3(1H- 1,2,4-triazol-1-yl)propyl]-4H-1,2,4-triazolium 749821-19-0 C13H14F2N7O+
Canagliflozin Defluoro Impurity Canagliflozin Defluoro Impurity
Dapagliflozin Impurity 6 Dapagliflozin Impurity 6
Imatinib Impurity 7 Imatinib Impurity 7 581076-64-4 C8H12N4
Nilotinib Impurity 1 Nilotinib Impurity 1 641571-05-3 C28H22F3N7O
Riociguat Impurtiy 1 Riociguat Impurtiy 1
Riociguat Impurtiy 6 Riociguat Impurtiy 6 1702271-98-4 C18H17FN8
Axitinib Impurity 4 Axitinib Impurity 4 1443118-73-7 C22H20N4OS
Brimonidine Impurity E Brimonidine Impurity E 168329-48-4 C9H8BrN5
Dapagliflozin Impurity G Dapagliflozin Impurity G
Dapoxetine Impurity B Dapoxetine Impurity B
Esomeprazole EP Impurity Esomeprazole EP Impurity
Fesoterodine Diol Dimer Fesoterodine Diol Dimer 1428856-45-4 C44H60N2O3
Gimeracil Impurity Gimeracil Impurity 17902-24-8 C8H9ClN2O3
Pramipexole Impurity H Pramipexole Impurity H
Quercetin 3-rutinoside 7-glucoside Quercetin 3-rutinoside 7-glucoside 30311-61-6 C33H40O21
Rivaroxaban Impurity H Rivaroxaban Impurity H 1807455-76-0 C25H24Cl2N4O6S2
Vonoprazan impurity A Vonoprazan impurity A
1-(4-(2-methyl-6-oxopiperidin-1-yl)phenyl)-3-morpholino-5,6-dihydropyridin-2(1H)-one 1-(4-(2-methyl-6-oxopiperidin-1-yl)phenyl)-3-morpholino-5,6-dihydropyridin-2(1H)-one 2098457-93-1 C21H27N3O3
5-ethyl 3-isopropyl 2-((2-aminoethoxy)methyl)-4-(2-chlorophenyl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 5-ethyl 3-isopropyl 2-((2-aminoethoxy)methyl)-4-(2-chlorophenyl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 2170716-02-4 C22H29ClN2O5
Acetamide,N-(2,3-dihydro-1,3-dioxo-1H-isoindol-4-yl)- Acetamide,N-(2,3-dihydro-1,3-dioxo-1H-isoindol-4-yl)- 6118-65-6 C10H8N2O3
Ibrutinib impurity D Ibrutinib impurity D
Mupirocin impurity A Mupirocin impurity A C26H44O9
Aurantio-obtusin-6-O-β-D-glucoside Aurantio-obtusin-6-O-β-D-glucoside 129025-96-3 C23H24O12
FG-4592 intermediate7 FG-4592 intermediate7 1509958-19-3 C20H20N2O4
Milnacipran Impurity 13 Milnacipran Impurity 13 710339-49-4 C12H16NO+
Ofloxacin/Levofloxacin Impurity A Ofloxacin/Levofloxacin Impurity A
Urapidil Impurity A Urapidil Impurity A
Tofacitinib Impurity I Tofacitinib Impurity I
N-(3-chloro-4-fluorophenyl)-6 ,7-dimethoxyquinazolin-4-amine N-(3-chloro-4-fluorophenyl)-6 ,7-dimethoxyquinazolin-4-amine 153437-78-6 C16H13ClFN3O2
Itopride Impurity C Itopride Impurity C 141996-97-6 C19H24N2O4
Mirabegron Impurity Mirabegron Impurity 1796931-48-0 C21H24N4O2S
34-Methylcyanocobalamin 34-Methylcyanocobalamin 38218-51-8 C64H90CoN14O14P
Nicorandil Impurity 1 Nicorandil Impurity 1
Piperacillin Impurity 6 Piperacillin Impurity 6
Bosutinib Impurity 5 Bosutinib Impurity 5 1391063-17-4 C26H29Cl2N5O3
Afatinib Impurity 14 Afatinib Impurity 14
methyl(E)-4-(methylamino)but-2-enoate methyl(E)-4-(methylamino)but-2-enoate 915788-22-6 C6H11NO2
methyl(E)-4-(methylamino)but-2-enoate methyl(E)-4-(methylamino)but-2-enoate 915788-22-6 C6H11NO2
Levomilnacipran  impurity Levomilnacipran impurity
Sofosbuvir IMpurity 4 Sofosbuvir IMpurity 4
1,3-bis(4-isobutylphenyl)butan-1-one 1,3-bis(4-isobutylphenyl)butan-1-one C24H32O
6-isobutyl-3-methyl-2,3-dihydro-1H-inden-1-one 6-isobutyl-3-methyl-2,3-dihydro-1H-inden-1-one 1340024-54-5 C14H18O
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