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61970-00-1
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???(??):
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LUCIFERASE
???(??):
RFLuc;Ultra-Glo;LUCIFERASE;EC 1.14.14.3;EC 1.13.12.7;Luciferase from;Einecs 263-359-4;Beetle luciferase;LUCIFERASE FIREFLY;FIREFLY LUCIFERASE
CBNumber:
CB3663963
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0
MOL ??:
Mol file

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111℃
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-20°C
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Specific Activity
15-30×106light units/mg protein
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  • ?? ? ?? ??
  • ?? ? ???? ?? (GHS)
??? ?? Xi,Xn,T
?? ???? ?? 36/37/38-22-52/53-45
????? 26-36/37-24/25-23-22-61-45-53
WGK ?? 3
F ?????? 3-10-21
????(GHS): GHS hazard pictogramsGHS hazard pictograms
?? ?: Warning
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?? ??·?? ?? ?? ?? ?? ?? ? ?? ?? P- ??
H302 ??? ??? ?? ?? ?? - ?? ?? 4 ?? GHS hazard pictograms P264, P270, P301+P312, P330, P501
H373 ??? ?? ?? ???? ??(??, ??? ?? ??? ?? ??? ??)? ??? ??? ? ?? ?? ???? ?? - ?? ?? ?? 2 ?? P260, P314, P501
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P260 ??·?·??·???·??·...·????? ???? ???.
P264 ?? ??? ?? ??? ????.
P264 ?? ??? ?? ??? ????.
P270 ? ??? ??? ??? ???, ???? ???? ???.
P301+P312 ??? ???? ??? ????(??)? ??? ????.
P314 ???? ??? ???? ??·??? ????.
P501 ...? ??? / ??? ?? ???.

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Luciferase (firefly recombinant) is a light-generating enzyme that catalyzes an oxidative reaction, using luciferin, ATP, and molecular oxygen to produce oxyluciferin, which yields visible light. The bioluminescence of this ATP-dependent luciferase is commonly used in reporter systems that are amenable to high-throughput screening.

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Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.

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Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.

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