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71418-44-5

中文名稱 熒光專用試劑
英文名稱 MONOBROMOBIMANE
CAS 71418-44-5
分子式 C10H11BrN2O2
分子量 271.11
MOL 文件 71418-44-5.mol
更新日期 2025/03/17 08:53:50
71418-44-5 結(jié)構(gòu)式 71418-44-5 結(jié)構(gòu)式

基本信息

中文別名
溴二胺
溴代雙滿
單溴代雙滿
熒光專用試劑
英文別名
MMB
MBBR
Nsc 608544
BROMOBIMANE
THIOLYTE(R) MB
MONOBROMOBIMANE
THIOLYTE(R) MB REAGENT
MBBr [MonobroMobiMane]
THIOLYTE MONOBROMOBIMANE REAGENT
MONOBROMOBIMANE, FOR FLUORESCENCE
所屬類別
生物化工:生化試劑

物理化學(xué)性質(zhì)

熔點(diǎn)152-154 °C(lit.)
沸點(diǎn)327.8±44.0 °C(Predicted)
密度1.66±0.1 g/cm3(Predicted)
儲(chǔ)存條件2-8°C
溶解度DMF: soluble
酸度系數(shù)(pKa)-3.36±0.70(Predicted)
形態(tài)粉末
顏色黃色
ε(消光系數(shù))4.6-5.1 at 396-398nm in H2O
最大波長(λmax)398 nm
BRN4430959
穩(wěn)定性感光
生物領(lǐng)域應(yīng)用Glutathione S-transferase substrates; detecting glutathione S-transferase,thiols,sulfite,homocysteine,mycothiol,sulfur compounds; thiol-reactive probes
CAS 數(shù)據(jù)庫71418-44-5

安全數(shù)據(jù)

安全說明22-24/25
WGK Germany3
F8
海關(guān)編碼2933.99.8290
熒光專用試劑價(jià)格(試劑級(jí))
報(bào)價(jià)日期產(chǎn)品編號(hào)產(chǎn)品名稱CAS號(hào)包裝價(jià)格
2025/02/05B4220溴代雙滿
Bromobimane
71418-44-520mg1330元
2025/02/05B4220溴代雙滿
Bromobimane
71418-44-5100mg3990元

常見問題列表

生物活性
Bromobimane基本上是非熒光物質(zhì),當(dāng)與小硫醇反應(yīng)時(shí),生成熒光產(chǎn)物。
體外研究

Bromobimanes in solution (aqueous or organic solvents of medium polarity) react with small thiols [e.g., the tripeptide thiol glutathione (GSH)], and with reactive protein thiol groups (e.g., hemoglobin). The reactions of bromobimanes with thiols are second order and dependent on pH, the active nucleophile being the thiolate anion. The reaction of bromobimane with a thiolate converts the nonfluorescent agent into water-soluble fluorescent products. The neutral agents mBBr and bBBr are moderately soluble in mediumpolarity organic solvents (acetonitrile, dichloromethane), and slightly soluble in water. The quaternary salt, qBBr, and the anionic bromobimane, SBBr, are soluble in water, but less soluble in organic solvents. Bromobimanes are yellow. The highly selective, rapid reactivity of bromobimanes toward thiols, the stability and fluorescence yield of the thiol derivatives, the ease of separation of the derivatives by reversed-phase HPLC, and the availability of both cell-penetrating and nonpenetrating forms, make the use bromobimanes an extremely powerful approach to the analysis of low molecular weight biothiols.

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