Isonipecotic acid synthesis
- Product Name:Isonipecotic acid
- CAS Number:498-94-2
- Molecular formula:C6H11NO2
- Molecular Weight:129.16
39546-32-2
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Yield:-
Reaction Conditions:
with amidase from Cupriavidus necator JMP134;water in aq. phosphate buffer at 30; pH=7;Enzymatic reaction;stereoselective reaction;
Steps:
2.4. Enzyme assay
General procedure: During the purification and characterization of amidase from Cupriavidus sp. KNK-J915, an enzyme assay was performed with (R,S)-BNPD as a substrate. The standard reaction mixture (0.2 mL) contained 100 mM potassium phosphate buffer (pH 7.0), 45.8 mM BNPD, and an appropriate amount of the enzyme. After the reaction was performed at 30°C for 0.5-1 h, the amount of BNPA was determined using HPLC. One unit of the enzyme was defined as the amount catalyzing the formation of 1 mol of BNPA per minute under the aforementioned condition. Protein content was determined by the Bradford method [11] with BSA as a standard using a kit from Bio-Rad Laboratories Ltd. (Tokyo, Japan).
References:
Nojiri, Masutoshi;Taoka, Naoaki;Yasohara, Yoshihiko [Journal of Molecular Catalysis B: Enzymatic,2014,vol. 109,p. 136 - 142]
55-22-1
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498-94-2
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13602-12-5
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498-94-2
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3731-38-2
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10314-98-4
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498-94-2
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