靶點(diǎn):詳見(jiàn)說(shuō)明
濃度:1mg/ml
應(yīng)用范圍:WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test1
CC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復(fù))
宿主:Rabbit
適應(yīng)物種:Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit Guinea Pig
標(biāo)記物:可現(xiàn)標(biāo)
克隆性:單克隆
保存條件:Store at -20%C for one year.
形態(tài):凍干粉
亞型:IgG
免疫原:KLH conjugated synthetic peptide derived from human
規(guī)格:50ul-100ul
Anti-AKT1抗體Myc-Tag單克隆抗體
size
200ug/100ug/50ug
Source
Mouse
Immunogen
Synthetic Peptide
Purification
The antibody was afinity-purified from mouse ascites by afinity-chromatography using specific immunogen.
Specificity
The antibody can detect Myc fusion proteins.
Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Concentration
1 mg/ml
Storage 1 Stability
-20*C/1 year
Reactivity
N/A
Applications
WB, IP, IF
Dilution
Anti-AKT1抗體WB: 1:5000 IP: 1:200 IF: 1:1000
Myc-Tag Monoclonal Antibody Images
IP antibody use: 3ug Flag Mouse lgG1 per ml Lysate, WB 1: 5000 1) untransfected 293 cell lysate 2) transfected 293 cell
lysate with Myc-Tag fusion protein 3) IP (transfected 293+ normal Mouse lgG+Protein G agarose) 4) IP (transfected
293+anti- C-Myc m
IF analysis of 293 cells transfected with a Myc-tag protein,1:2000 dilution (blue DAPI, red anti-MyC)
1ug Myc fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000
Anti-AKT1抗體注意事項(xiàng):
1抗體保存原則:避免反復(fù)凍融
2:短期保存的抗體:如抗體幾周內(nèi)就會(huì)使用，收到后推薦在2-8°C保存，2-8*C短暫保存數(shù)周對(duì)抗體活性沒(méi)有影響。
3.長(zhǎng)期保存的抗體:如抗體需超過(guò)1個(gè)月后才會(huì)使用，請(qǐng)酌情分裝后于-20°C保存。
4建議:將抗體保存在冰箱里層，避免溫度變化造成反復(fù)凍融。
絕對(duì)避免將抗體保存在自動(dòng)除霜冰箱中。
反復(fù)凍融過(guò)程中，冰晶會(huì)對(duì)抗體的空間結(jié)構(gòu)造成破壞，導(dǎo)致蛋白變性形成多聚體，從而降低抗體的結(jié)合能力，加快抗體降解速度。