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     Western blot analysis of Histone H2B (formyl K116) expression in HeLa cell lysate.    

  
  

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     All lanes use the Antibody at 1:2k dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2k dilution for 1 hour at room temperature.    

  
  

Histone H2B(formylK116) antibodies are specialized tools used to study post-translational modifications (PTMs) of histone H2B, specifically formylation at lysine 116 (K116). Histones, including H2B, play critical roles in chromatin organization and gene regulation. PTMs like formylation alter histone function by modulating chromatin structure or recruiting effector proteins. The formyl group (-CHO) at K116 is a relatively understudied modification, potentially linked to DNA damage response, transcriptional regulation, or oxidative stress pathways. This modification might arise from non-enzymatic chemical reactions (e.g., reactive oxygen species) or enzymatic processes, though its exact mechanism remains unclear.

Antibodies targeting formylK116 are essential for detecting this modification in experimental settings, such as Western blotting, immunofluorescence, or chromatin immunoprecipitation (ChIP). They help researchers investigate its biological significance, including potential roles in genome stability, epigenetic signaling, or disease states like cancer. For example, aberrant histone formylation could influence chromatin dynamics in tumors or inflammatory conditions. However, cross-reactivity with similar PTMs (e.g., acetylation or methylation) must be ruled out during validation. Studies using these antibodies contribute to understanding how non-canonical histone modifications fine-tune cellular responses, bridging gaps between chromatin biology and metabolic or stress-related pathways.