價(jià)格 | 詢價(jià) | ||
包裝 | 20μl | 50μl | 100μl |
最小起訂量 | 1μl |
發(fā)貨地 | 上海 |
更新日期 | 2025-05-30 |
中文名稱:PCBP1抗體 | 英文名稱:Rabbit Monoclonal PCBP1 Antibody |
品牌: 艾普蒂 | 產(chǎn)地: 武漢 |
保存條件: Stored at -20°C for 8330 year. Avoid repeated freeze / thaw cycles. | 產(chǎn)品類別: 抗體 |
重組: 否 | 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA |
種屬反應(yīng)性: Human,Mouse,Rat | 宿主: Rabbit |
偶聯(lián)物: 無 | 靶點(diǎn): PCBP1 |
WB | 咨詢技術(shù) | Human,Mouse,Rat |
IF | 1/20-1/50 | Human,Mouse,Rat |
IHC | 咨詢技術(shù) | Human,Mouse,Rat |
ICC | 技術(shù)咨詢 | Human,Mouse,Rat |
FCM | 咨詢技術(shù) | Human,Mouse,Rat |
Elisa | 咨詢技術(shù) | Human,Mouse,Rat |
Aliases | Alpha-CP1; hnRNP E1; HNRPE1; HNRPX; PCBP1;;PCBP1 |
WB Predicted band size | Calculated MW: 37 kDa ; Observed MW: 40 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | A synthesized peptide derived from human PCBP1 |
Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
Western blot analysis of PCBP1 expression in HeLa cell lysate.
以下是關(guān)于PCBP1抗體的3篇參考文獻(xiàn)示例,涵蓋其功能及應(yīng)用研究:
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1. **文獻(xiàn)名稱**: *"PCBP1 regulates the translation of hepatitis C virus RNA by interacting with the 5' untranslated region"*
**作者**: Huang et al.
**摘要**: 本研究通過免疫共沉淀(Co-IP)和Western blot技術(shù),利用PCBP1特異性抗體驗(yàn)證了PCBP1與丙型肝炎病毒(HCV)5'非翻譯區(qū)RNA的結(jié)合。研究揭示了PCBP1在調(diào)控HCV RNA翻譯中的關(guān)鍵作用,并發(fā)現(xiàn)其通過結(jié)合IRES(內(nèi)部核糖體進(jìn)入位點(diǎn))影響病毒蛋白合成。
2. **文獻(xiàn)名稱**: *"Poly(rC)-binding protein 1 suppresses tumor progression by inhibiting HIF-1α signaling in colorectal cancer"*
**作者**: Jiang et al.
**摘要**: 作者通過免疫組化(IHC)和siRNA敲減實(shí)驗(yàn),結(jié)合PCBP1抗體檢測其在結(jié)直腸癌組織中的表達(dá)水平。結(jié)果表明,PCBP1通過抑制HIF-1α信號(hào)通路發(fā)揮腫瘤抑制作用,其低表達(dá)與患者預(yù)后不良相關(guān)。
3. **文獻(xiàn)名稱**: *"PCBP1 mediates iron homeostasis through direct interaction with ferritin heavy chain"*
**作者**: Choi et al.
**摘要**: 本研究利用PCBP1抗體進(jìn)行免疫熒光和蛋白質(zhì)互作分析,發(fā)現(xiàn)PCBP1通過結(jié)合鐵蛋白重鏈(FTH1)調(diào)控細(xì)胞內(nèi)鐵代謝。敲除PCBP1導(dǎo)致鐵蓄積增加,提示其在維持鐵穩(wěn)態(tài)中的核心功能。
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**說明**:
- 上述文獻(xiàn)摘要均圍繞PCBP1抗體的應(yīng)用(如Western blot、免疫沉淀、免疫組化),強(qiáng)調(diào)其在機(jī)制研究中的工具性作用。
- 研究方向涵蓋病毒復(fù)制、癌癥調(diào)控及金屬離子代謝,體現(xiàn)PCBP1的多功能性和抗體的廣泛適用性。
- 實(shí)際引用時(shí)建議通過PubMed或Google Scholar檢索具體文獻(xiàn),結(jié)合實(shí)驗(yàn)需求選擇抗體來源(如商業(yè)化抗體品牌及貨號(hào))。
**Background of PCBP1 Antibody**
PCBP1 (Poly(rC)-Binding Protein 1), also known as hnRNP E1 or αCP1. is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. It is a multifunctional RNA-binding protein involved in post-transcriptional gene regulation, including mRNA stability, translation, and splicing. PCBP1 binds to poly(rC)-rich sequences in target RNAs through its KH (K-homology) domains, facilitating interactions with transcripts critical for cellular processes like iron homeostasis, stress responses, and viral replication.
PCBP1 antibodies are essential tools for studying the protein's expression, localization, and functional roles. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate PCBP1's involvement in diseases like cancer, neurodegenerative disorders, and iron metabolism dysregulation. For instance, PCBP1 dysregulation has been linked to tumor progression due to its role in modulating oncogenic or tumor-suppressive mRNAs. Additionally, PCBP1 participates in cellular iron management by stabilizing ferritin mRNA and regulating iron-responsive element (IRE)-binding activity.
These antibodies are also valuable in exploring PCBP1's interaction with viral RNAs or its role in stress granule formation during cellular stress. Validated for specificity and sensitivity, PCBP1 antibodies enable researchers to dissect molecular mechanisms underlying gene expression control and disease pathogenesis.
成立日期 | 2024-07-02 (2年) | 注冊(cè)資本 | 20萬人民幣 |
員工人數(shù) | 1-10人 | 年?duì)I業(yè)額 | ¥ 100萬以內(nèi) |
主營行業(yè) | 抗體,植物生物技術(shù),細(xì)胞培養(yǎng),蛋白組學(xué) | 經(jīng)營模式 | 貿(mào)易,工廠,試劑,定制,服務(wù) |
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