The guanylate kinase 1 (GUK1) antibody is a tool used to detect and study GUK1. a conserved enzyme critical for nucleotide metabolism. GUK1 catalyzes the ATP-dependent phosphorylation of GMP to GDP, a rate-limiting step in the cGMP-dependent signaling pathway and nucleotide synthesis. It plays a vital role in RNA/DNA biosynthesis, cell cycle regulation, and mitochondrial function. GUK1 is ubiquitously expressed, localized in the cytoplasm and mitochondria, and encoded by the *GUK1* gene in humans.
GUK1 antibodies are widely utilized in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate GUK1's expression, localization, and interactions in cellular models or tissues. Research highlights its involvement in diseases, including cancers (e.g., colorectal, hepatocellular carcinoma) where dysregulated GUK1 correlates with proliferation, and neurological disorders linked to mitochondrial dysfunction. Additionally, GUK1 interacts with proteins like postsynaptic density components, suggesting roles in neurodevelopment.
These antibodies aid in exploring GUK1’s molecular mechanisms, therapeutic potential, and biomarker utility. Validation for specificity, cross-reactivity, and application compatibility is essential for experimental accuracy. Ongoing studies focus on its dual metabolic and structural roles, particularly in cellular energy balance and disease pathogenesis.