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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: LOVO cell lysate, Primary antibody: P11723(DNASE2 Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 2 minutes    

  
  

**Background of DNASE2 Antibodies**

DNASE2 (Deoxyribonuclease II), also known as Acid DNase, is a lysosomal endonuclease responsible for degrading DNA during apoptosis and phagocytosis. It functions optimally in acidic environments, cleaving DNA into oligonucleotides without requiring divalent cations. DNASE2 plays critical roles in removing nuclear DNA from erythroid precursor cells, maintaining immune tolerance, and processing extracellular DNA from pathogens or dead cells.

Antibodies targeting DNASE2 are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to quantify protein levels or visualize tissue distribution. Dysregulation of DNASE2 has been linked to autoimmune diseases (e.g., systemic lupus erythematosus) due to impaired clearance of self-DNA, chronic inflammation, and certain cancers. Antibodies help investigate these associations by detecting aberrant DNASE2 expression or activity.

Research also explores DNASE2's role in age-related macular degeneration (AMD) and neurodegenerative disorders, where defective DNA degradation may contribute to pathology. Additionally, DNASE2 antibodies aid in distinguishing between isoforms (DNASE2α and DNASE2β) and validating gene-editing outcomes in cellular models. Commercial DNASE2 antibodies are typically raised in rabbits or mice, validated for specificity using knockout controls. Their applications extend to biomarker discovery and therapeutic target validation, underscoring their importance in understanding DNASE2-related mechanisms in health and disease.