The NUF2 (Ndc80 kinetochore complex component) antibody is a crucial tool for studying the role of the NUF2 protein in cellular processes, particularly during mitosis. NUF2 is a core component of the Ndc80 complex, which is essential for proper chromosome segregation by mediating kinetochore-microtubule interactions. This complex ensures accurate alignment and separation of sister chromatids, maintaining genomic stability. Dysregulation of NUF2 has been implicated in mitotic errors, aneuploidy, and cancer progression, as overexpression is observed in various malignancies.
NUF2 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression levels, subcellular localization, and interactions within the Ndc80 complex. Researchers employ these antibodies to investigate NUF2's regulatory mechanisms in cell cycle checkpoints, its role in cancer pathogenesis, and its potential as a therapeutic target or prognostic biomarker.
Studies using NUF2 antibodies have highlighted its importance in spindle assembly checkpoint signaling and response to anti-mitotic drugs. Additionally, they help explore correlations between NUF2 dysregulation and tumor aggressiveness, metastasis, or drug resistance. The antibody's specificity and reliability make it indispensable for unraveling mitotic mechanisms and developing targeted cancer therapies.