中文名稱:COPE抗體 | 英文名稱:Rabbit Polyclonal COPE Antibody |
品牌: 艾普蒂 | 產(chǎn)地: 武漢 |
保存條件: Stored at -20°C for 4901 year. Avoid repeated freeze / thaw cycles. | 產(chǎn)品類別: 抗體 |
重組: 否 | 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA |
種屬反應(yīng)性: Human,Mouse,Rat | 宿主: Rabbit |
偶聯(lián)物: 無 | 靶點: COPE |
WB | 咨詢技術(shù) | Human,Mouse,Rat |
IF | 咨詢技術(shù) | Human,Mouse,Rat |
IHC | 1/20-1/100 | Human,Mouse,Rat |
ICC | 技術(shù)咨詢 | Human,Mouse,Rat |
FCM | 咨詢技術(shù) | Human,Mouse,Rat |
Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
Aliases | epsilon-COP |
WB Predicted band size | 34 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse |
Immunogen | Fusion protein of human COPE |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Hela, A375 and PC-3 cell lysates, Primary antibody: P09543(COPE Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P09543(COPE Antibody) at dilution 1/25. (Original magnification: ×200)
以下是關(guān)于COPE抗體的3篇示例參考文獻(內(nèi)容為模擬生成,建議通過學(xué)術(shù)數(shù)據(jù)庫核實最新文獻):
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1. **文獻名稱**: "Structural and functional analysis of the COPE subunit in the COPI vesicle complex"
**作者**: Duden, R., et al.
**摘要**: 研究利用X射線晶體學(xué)解析了COPE亞基的三維結(jié)構(gòu),揭示了其在COPI囊泡組裝中的關(guān)鍵作用,并證明其與β'-COP的結(jié)合對維持復(fù)合體穩(wěn)定性至關(guān)重要。
2. **文獻名稱**: "COPE antibody-based detection of coatomer dynamics in intracellular trafficking"
**作者**: Hsu, V.W., et al.
**摘要**: 開發(fā)了一種特異性識別COPE的單克隆抗體,用于追蹤COPI復(fù)合體在高爾基體-內(nèi)質(zhì)網(wǎng)運輸中的動態(tài)變化,證實COPE缺失會導(dǎo)致分泌途徑異常。
3. **文獻名稱**: "Dysregulation of COPE in cancer: Implications for tumor progression"
**作者**: Tanabe, K., et al.
**摘要**: 通過免疫組化分析發(fā)現(xiàn),COPE在多種癌細(xì)胞中表達(dá)異常,其抗體標(biāo)記顯示COPE下調(diào)與細(xì)胞分泌蛋白功能障礙及腫瘤轉(zhuǎn)移相關(guān)。
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**注**:以上文獻信息為示例性質(zhì),實際研究中請通過PubMed、Google Scholar等平臺檢索關(guān)鍵詞(如“COPE antibody”“coatomer epsilon”)獲取準(zhǔn)確文獻。
COPE antibodies target the ε-subunit of the COPI (coatomer protein complex I) complex, a key player in intracellular vesicular transport. The COPI complex, composed of seven subunits (α, β, β', γ, δ, ε, ζ), is primarily involved in retrograde trafficking within the Golgi apparatus and between the Golgi and the endoplasmic reticulum (ER). This trafficking ensures proper protein sorting, organelle maintenance, and cellular homeostasis. The ε-subunit (COPE) contributes to coatomer assembly and vesicle budding, playing a critical role in maintaining ER-Golgi architecture and regulating cargo selection.
COPE antibodies are widely used in cell biology research to study COPI-mediated transport mechanisms, protein localization, and organelle dynamics. They enable detection of COPE expression levels via techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding in investigations of secretory pathway dysregulation. Such studies are crucial for understanding diseases linked to COPI dysfunction, including certain cancers, neurodegenerative disorders (e.g., Alzheimer’s), and congenital glycosylation defects. Additionally, COPE antibodies help explore COPΙ's role in lipid homeostasis and autophagy. Their application in model organisms and cell lines has provided insights into evolutionary conservation of vesicular transport systems. By elucidating COPI-related mechanisms, these antibodies contribute to therapeutic strategies targeting membrane trafficking anomalies.
成立日期 | 2024-07-02 (1年) | 注冊資本 | 20萬人民幣 |
員工人數(shù) | 1-10人 | 年營業(yè)額 | ¥ 100萬以內(nèi) |
主營行業(yè) | 抗體,蛋白組學(xué),細(xì)胞生物學(xué) | 經(jīng)營模式 | 貿(mào)易,工廠,試劑,定制,服務(wù) |
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