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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using HSP70 mouse mAb against NIH/3T3 (1), Hela (2), HepG2 (3), Hek293 (4), COS-7 (5), A549 (6), and Jurkat (7) cell lysate.    

  
  

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     Flow cytometric analysis of Hela cells using HSP70 mouse mAb (green) and negative control (red).    

  
  

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     Flow cytometric analysis of Jurkat cells using HSP70 mouse mAb (green) and negative control (red).    

  
  

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     Flow cytometric analysis of Raji cells using HSP70 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using HSP70 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using HSP70 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using HSP70 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using HSP70 mouse mAb with DAB staining.    

  
  

HSP70 antibodies are essential tools for studying the 70-kilodalton heat shock protein family (HSP70), a group of evolutionarily conserved molecular chaperones critical for protein homeostasis. HSP70 proteins, including stress-inducible forms like HSPA1A and constitutive isoforms like HSPA8. assist in protein folding, prevent aggregation under cellular stress, and facilitate substrate trafficking across membranes. These functions make HSP70 pivotal in diverse physiological and pathological processes, including neurodegeneration, cancer, inflammation, and immune responses.

Antibodies targeting HSP70 are widely employed in research to investigate its expression patterns, subcellular localization, and interactions. Commercial HSP70 antibodies are typically raised against specific epitopes in conserved regions, though selectivity between isoforms (e.g., distinguishing inducible vs. constitutive forms) varies depending on immunogen design. Validation remains crucial due to sequence homology among family members and cross-reactivity risks. Applications span Western blotting, immunohistochemistry, immunoprecipitation, and flow cytometry. In clinical contexts, HSP70 antibodies aid in exploring its role as a biomarker for cancer prognosis or stress responses. Recent interest focuses on its extracellular roles in immune modulation and as a potential therapeutic target. Researchers must carefully select antibodies based on target isoforms, post-translational modifications, and experimental contexts to ensure specificity.