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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Jurkat cells, Hela cells, Primary antibody: P08494(BUB3 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    

  
  

BUB3 antibodies are essential tools for studying the spindle assembly checkpoint (SAC), a critical surveillance mechanism ensuring accurate chromosome segregation during mitosis. BUB3. a conserved SAC protein, forms complexes with BUB1 and BUBR1 to monitor microtubule-kinetochore attachment. It acts as a scaffold, facilitating protein interactions and signal transduction that delay anaphase until all chromosomes achieve proper bipolar attachment. Dysregulation of BUB3 is linked to chromosomal instability, aneuploidy, and cancer progression.

Researchers use BUB3 antibodies to investigate its expression levels, subcellular localization, and interactions in normal and pathological contexts, such as tumorigenesis or chemotherapy resistance. These antibodies are widely applied in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specificity validation via knockout/knockdown controls is crucial, as off-target reactivity may compromise data reliability. Recent studies also explore BUB3's non-mitotic roles, including DNA damage response and immune regulation, expanding its relevance beyond cell cycle research. Commercial BUB3 antibodies are typically raised against recombinant human protein fragments, with rabbit and mouse being common host species. Proper antibody selection and experimental optimization remain key to elucidating BUB3's multifaceted functions in health and disease.