同用名/別名（Synonym）
IL-12; Interleukin 12; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40
描述（Description）
IL-12 beta Protein, also known as natural killer cell stimulatory factor 2, is a common subunit (p40) of IL-12 and IL-23. IL-12 is a inflammatory factor expressed by activated macrophages, and involves in Th1-type immune response against cancer[1]. IL-12 beta Protein located outside the cell membrane, involves in singalling mediated by Jak-STAT[3]. IL-12 beta Protein consists of 328 amino acids (M1-S328) with a fibronectin type-III domain (237-328 a.a). IL-12 beta Protein, Human (M1-S328) is produced in HEK293 cells with a C-Terminal Fc-tag.
純度（Purity）
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
生物活性（Bioactivity）
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is ＜0.5 ng/mL.
氨基酸序列（Target Protein Sequence）
IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS&RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTSFMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNAS
制劑（Trait）
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
復(fù)溶（Reconstitution）
It is recommended to redissolve in sterile deionized water.
保存條件&效期（Storage & Stability）
36 months at 2°C to 8°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
運(yùn)輸方式(Shipping)
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
生物活性（Bioactivity） | 
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is ＜0.5 ng/mL. |
電泳（SDS-PAGE） | 
Greater than 95% as determined by reducing SDS-PAGE. |
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關(guān)鍵字： GMP級(jí);高純度;批間一致;生物活性;科研專供;
在生物科技領(lǐng)域，豐度蛋白專注重組蛋白供應(yīng)，以“豐度的風(fēng)帆為蛋白提供動(dòng)力，豐度為蛋白護(hù)航”為使命，賦能生命科學(xué)發(fā)展。
蘇州豐度依托先進(jìn)技術(shù)平臺(tái)，打造成熟生產(chǎn)體系，嚴(yán)控基因工程設(shè)計(jì)、細(xì)胞培養(yǎng)、蛋白純化等環(huán)節(jié)，優(yōu)化工藝與管理，確保產(chǎn)品高純度、高活性且批間一致。產(chǎn)品覆蓋基礎(chǔ)研究、生物醫(yī)藥研發(fā)等多領(lǐng)域，為科研及生產(chǎn)提供多樣、適配產(chǎn)品，助力突破技術(shù)難題 。