中文名稱:CHMP1A抗體 | 英文名稱:Rabbit Polyclonal CHMP1A Antibody |
品牌: 艾普蒂 | 產(chǎn)地: 武漢 |
保存條件: Stored at -20°C for 1890 year. Avoid repeated freeze / thaw cycles. | 產(chǎn)品類別: 抗體 |
重組: 否 | 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA |
種屬反應(yīng)性: Human,Mouse,Rat | 宿主: Rabbit |
偶聯(lián)物: 無 | 靶點(diǎn): CHMP1A |
WB | 咨詢技術(shù) | Human,Mouse,Rat |
IF | 咨詢技術(shù) | Human,Mouse,Rat |
IHC | 1/50-1/200 | Human,Mouse,Rat |
ICC | 技術(shù)咨詢 | Human,Mouse,Rat |
FCM | 咨詢技術(shù) | Human,Mouse,Rat |
Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
Aliases | Proenkephalin-A, Synenkephalin, Met-enkephalin, Opioid growth factor, OGF, PENK(114-133), PENK(143-183), Met-enkephalin-Arg-Gly-Leu, Leu-enkephalin, PENK(237-258), Met-enkephalin-Arg-Phe, PENK |
Entrez GeneID | 5179 |
WB Predicted band size | 30.8kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This PENK antibody is generated from a mouse immunized with a recombinant protein from the human region of human PENK. |
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All lanes : Anti-PENK Antibody at 1:2000 dilution Lane 1: T47D whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Mouse IgG/A/M(H/L), Peroxidase conjugated at 1/2000 dilution. Observed band size : 35kDa Blocking/Dilution buffer: 5% NFDM/TBST.
以下是關(guān)于CHMP1A抗體的3篇參考文獻(xiàn)及其簡(jiǎn)要摘要:
1. **文獻(xiàn)名稱**: *CHMP1A regulates tumorigenesis and cell sensitivity to radiation through the NF-κB pathway in human glioma*
**作者**: Li X, et al.
**摘要**: 本研究利用CHMP1A抗體通過Western blot和免疫組化分析,發(fā)現(xiàn)CHMP1A在膠質(zhì)瘤中表達(dá)下調(diào),并通過調(diào)控NF-κB通路影響腫瘤生長(zhǎng)和放射敏感性。
2. **文獻(xiàn)名稱**: *CHMP1A interacts with ESCRT-III to regulate endosomal sorting and miRNA silencing*
**作者**: Schmidt C, et al.
**摘要**: 通過免疫共沉淀和免疫熒光技術(shù)(使用CHMP1A抗體),揭示了CHMP1A與ESCRT-III復(fù)合物相互作用,調(diào)控內(nèi)體分選及microRNA沉默功能,影響細(xì)胞膜修復(fù)過程。
3. **文獻(xiàn)名稱**: *Loss of CHMP1A disrupts neural progenitor proliferation and causes microcephaly in mice*
**作者**: Muzio L, et al.
**摘要**: 研究通過CHMP1A抗體的免疫染色發(fā)現(xiàn),CHMP1A缺失導(dǎo)致小鼠神經(jīng)前體細(xì)胞增殖異常和小頭畸形,證實(shí)其在中樞神經(jīng)系統(tǒng)發(fā)育中的關(guān)鍵作用。
(注:以上文獻(xiàn)信息為模擬示例,實(shí)際引用請(qǐng)以具體論文內(nèi)容為準(zhǔn)。)
CHMP1A (Charged Multivesicular Body Protein 1A) is a key component of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery, which plays essential roles in membrane remodeling processes, including multivesicular body (MVB) formation, cytokinesis, viral budding, and autophagy. As part of the ESCRT-III subcomplex, CHMP1A facilitates membrane scission by assembling into helical filaments that constrict membranes. Dysregulation of CHMP1A is linked to neurological disorders, cancer, and developmental defects, underscoring its importance in cellular homeostasis.
CHMP1A antibodies are critical tools for studying its expression, localization, and function. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect CHMP1A in various biological samples. Validated antibodies typically show specificity for CHMP1A (≈25 kDa) through knockout cell line controls, distinguishing it from homologous ESCRT-III proteins. Researchers utilize CHMP1A antibodies to explore its role in ESCRT-mediated processes, such as exosome secretion, nuclear envelope repair, and cell division. Notably, CHMP1A mutations have been associated with microcephaly and pontocerebellar hypoplasia, making these antibodies valuable in neurodevelopmental disease research. Commercial CHMP1A antibodies are available as monoclonal or polyclonal formats, often raised against conserved epitopes in human, mouse, or rat homologs. Proper validation using orthogonal methods remains crucial, as cross-reactivity with other CHMP family members (e.g., CHMP1B) can occur. Recent studies also employ CHMP1A antibodies to investigate its non-canonical roles in chromatin remodeling and TGF-β signaling.
成立日期 | 2024-06-24 (1年) | 注冊(cè)資本 | 50萬(元) |
員工人數(shù) | 1-10人 | 年?duì)I業(yè)額 | ¥ 100萬以內(nèi) |
主營(yíng)行業(yè) | 中間體,化學(xué)試劑 | 經(jīng)營(yíng)模式 | 貿(mào)易,工廠,試劑,定制,服務(wù) |
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