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MET抗體,Mouse Monoclonal MET Antibody
  • MET抗體,Mouse Monoclonal MET Antibody
  • MET抗體,Mouse Monoclonal MET Antibody
  • MET抗體,Mouse Monoclonal MET Antibody

MET抗體

價(jià)格 詢(xún)價(jià)
包裝 20μl 50μl 100μl
最小起訂量 1μl
發(fā)貨地 湖北
更新日期 2025-05-10
QQ交談 微信洽談

產(chǎn)品詳情

中文名稱(chēng):MET抗體英文名稱(chēng):Mouse Monoclonal MET Antibody
品牌: 艾普蒂產(chǎn)地: 武漢
保存條件: Stored at -20°C for 901 year. Avoid repeated freeze / thaw cycles.產(chǎn)品類(lèi)別: 抗體
重組: 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA
種屬反應(yīng)性: Human,Mouse,Rat 宿主: Mouse
偶聯(lián)物: 無(wú)靶點(diǎn): MET
2025-05-10 MET抗體 Mouse Monoclonal MET Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武漢 Stored at -20°C for 901 year. Avoid repeated freeze / thaw cycles. 抗體

驗(yàn)證與應(yīng)用

應(yīng)用及物種
WB咨詢(xún)技術(shù) Human,Monkey
IF咨詢(xún)技術(shù) Human,Monkey
IHC1/200 - 1/1000 Human,Monkey
ICC1/200 - 1/1000 Human,Monkey
FCM1/200 - 1/400 Human,Monkey
Elisa1/10000 Human,Monkey
   

產(chǎn)品詳情

AliasesHGFR; AUTS9; RCCP2; c-Met; DFNB97
Entrez GeneID4233
clone5E9C4
WB Predicted band size155kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Monkey
ImmunogenPurified recombinant fragment of human MET (AA: 743-932) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Western blot analysis using MET mouse mAb against A549 (1), COS7 (2), Hela (3), HEK293 (4), HepG2 (5), and A431 (6) cell lysate.    


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       Immunofluorescence analysis of Hela cells using MET mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of MCF-7 cells using MET mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using MET mouse mAb with DAB staining.    


           

參考文獻(xiàn)

以下是3-4篇關(guān)于MET抗體的代表性文獻(xiàn)及其摘要概括:

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1. **文獻(xiàn)名稱(chēng)**: *Onartuzumab in combination with erlotinib in advanced non-small cell lung cancer*

**作者**: Spigel DR et al.

**摘要**: 該研究評(píng)估MET單抗Onartuzumab聯(lián)合厄洛替尼治療晚期非小細(xì)胞肺癌(NSCLC)的III期臨床試驗(yàn)。結(jié)果顯示,與安慰劑組相比,聯(lián)合治療未能顯著改善患者總生存期或無(wú)進(jìn)展生存期,提示MET靶向治療需精準(zhǔn)篩選生物標(biāo)志物陽(yáng)性患者。

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2. **文獻(xiàn)名稱(chēng)**: *SAIT301. a humanized anti-MET antibody, suppresses tumor growth in MET-amplified gastric cancer models*

**作者**: Jin H et al.

**摘要**: 本研究報(bào)道了人源化MET單抗SAIT301在MET擴(kuò)增胃癌模型中的療效。SAIT301通過(guò)阻斷HGF/MET信號(hào)通路并誘導(dǎo)MET內(nèi)吞降解,顯著抑制腫瘤生長(zhǎng),為MET依賴(lài)性腫瘤的靶向治療提供了臨床前證據(jù)。

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3. **文獻(xiàn)名稱(chēng)**: *Sym015: A MET antibody mixture with enhanced efficacy in models of MET-amplified cancers*

**作者**: Kragh M et al.

**摘要**: 研究開(kāi)發(fā)了針對(duì)MET不同表位的雙抗體混合物Sym015.其在MET擴(kuò)增或exon 14跳躍突變的腫瘤模型中表現(xiàn)出協(xié)同作用,可克服單抗耐藥性并顯著抑制腫瘤生長(zhǎng),支持其作為廣譜MET靶向治療的潛力。

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4. **文獻(xiàn)名稱(chēng)**: *MET-targeted antibody resistance mediated by HER kinase activation*

**作者**: Mazieres J et al.

**摘要**: 該文獻(xiàn)揭示了MET單抗治療小細(xì)胞肺癌(SCLC)的耐藥機(jī)制。研究發(fā)現(xiàn),HER家族激酶(如EGFR、HER3)的代償性激活可導(dǎo)致MET抗體療效下降,提示聯(lián)合抑制HER/MET信號(hào)通路可能延緩耐藥。

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以上文獻(xiàn)涵蓋MET抗體在臨床前研究、臨床試驗(yàn)及耐藥機(jī)制中的關(guān)鍵進(jìn)展,反映了該領(lǐng)域的挑戰(zhàn)與潛在策略。

       

背景信息

MET antibodies are therapeutic or diagnostic agents targeting the MET receptor, a tyrosine kinase receptor encoded by the *MET* proto-oncogene. MET, also known as hepatocyte growth factor (HGF) receptor, plays a critical role in embryogenesis, tissue repair, and cancer progression by regulating cell proliferation, survival, motility, and angiogenesis. Aberrant MET signaling, caused by gene amplification, mutations, or HGF overexpression, is implicated in tumor growth, metastasis, and resistance to therapies in various cancers, including lung, gastric, and hepatocellular carcinomas.

Therapeutic MET antibodies are designed to block HGF binding or receptor dimerization, inhibiting downstream signaling. Examples include monovalent monoclonal antibodies (e.g., onartuzumab) and bivalent antibodies (e.g., emibetuzumab). While early clinical trials showed mixed results—onartuzumab failed in phase III trials for non-small cell lung cancer (NSCLC)—renewed interest focuses on patient stratification using biomarkers (e.g., *MET* amplification/exon 14 skipping) and combination therapies. Bispecific antibodies targeting MET alongside other receptors (e.g., EGFR or VEGF) aim to enhance efficacy and overcome resistance.

Diagnostic MET antibodies aid in detecting MET overexpression or activation in tumors, guiding targeted therapy decisions. Challenges include tumor heterogeneity, limited predictive biomarkers, and compensatory signaling. Ongoing research explores next-generation antibodies, antibody-drug conjugates, and immunotherapies to optimize MET-targeted strategies in precision oncology.

       
關(guān)鍵字: MET抗體;MET;MET Antibody;

公司簡(jiǎn)介

湖北瑞和寧生物科技有限公司研究創(chuàng)新能力, 擁有完整、科學(xué)、質(zhì)量管理體系。并不斷致力于技術(shù)創(chuàng)新,產(chǎn)品創(chuàng)新和管理創(chuàng)新,贏得了國(guó)內(nèi)外廣大用戶(hù)的信任。
成立日期 2024-06-24 (2年) 注冊(cè)資本 50萬(wàn)(元)
員工人數(shù) 1-10人 年?duì)I業(yè)額 ¥ 100萬(wàn)以?xún)?nèi)
主營(yíng)行業(yè) 中間體,化學(xué)試劑 經(jīng)營(yíng)模式 貿(mào)易,工廠,試劑,定制,服務(wù)
  • 湖北瑞和寧生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注冊(cè)資本:50萬(wàn)(元)
  • 企業(yè)類(lèi)型:有限責(zé)任公司(自然人投資或控股)
  • 主營(yíng)產(chǎn)品:中間體。試劑
  • 公司地址:江漢區(qū)
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