Mouse B7-2 / CD86 Protein, His Tag (MALS verified)

分子別名（Synonym）

CD86,B7-2,B70,CD28LG2,LAB72,MGC34413

表達區(qū)間及表達系統(tǒng)（Source）

Mouse B7-2, His Tag (CD6-M52H0) is expressed from human 293 cells (HEK293). It contains AA Val 24 - Glu 245 (Accession # NP_062261.3).

Predicted N-terminus: Val 24

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 27.1 kDa. The protein migrates as 40-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

分化簇86（CD86）也稱為B淋巴細胞活化抗原B7-2，是一種I型膜蛋白，是免疫球蛋白超家族的成員，在交叉樹突細胞、朗格罕斯細胞、外周血樹突細胞、記憶B細胞和生發(fā)中心B細胞上組成型表達。此外，B72在單核細胞上的表達水平較低，可以通過干擾素γ上調(diào)。CD86是T細胞表面兩種不同蛋白質(zhì)的配體：CD28（用于自動調(diào)節(jié)和細胞間結(jié)合）和CTLA-4（用于減弱調(diào)節(jié)和細胞分離）。CD86與CD80協(xié)同作用以激活T細胞。最近的研究表明，B7-2促進了成熟APC庫的產(chǎn)生，并促進了APC的功能和存活。此外，B7蛋白還通過激活巨噬細胞中的NF-κB信號通路參與先天免疫反應(yīng)。因此，CD86被視為免疫治療的有前景的候選者?；羝娼鹆馨土龌颊叩?/span>CD86+巨噬細胞是一線治療潛在無反應(yīng)的獨立標志物。