Human EGF Protein, His Tag, premium grade

分子別名（Synonym）

EGF,URG,HOMG4

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human EGF Protein, His Tag, premium grade (EGF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1).

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

無菌（Sterility）

Negative

支原體（Mycoplasma）

Negative.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

人表皮生長因子（EGF）也稱為HOMG4和URG，是一種生長因子，通過與其受體EGFR結(jié)合在調(diào)節(jié)細(xì)胞生長，增殖和分化中起重要作用。表皮生長因子可以在人血小板，巨噬細(xì)胞，尿液，唾液，牛奶和血漿中發(fā)現(xiàn)。EGF是EGF蛋白家族的創(chuàng)始成員。所有家族成員都包含一個(gè)或多個(gè)保守氨基酸序列的重復(fù)序列。唾液EGF的生物學(xué)效應(yīng)包括口腔和胃食管潰瘍的愈合，胃酸分泌的抑制，D合成的刺激以及粘膜保護(hù)免受腔內(nèi)有害因素（如胃酸，膽汁酸，胃蛋白酶和胰蛋白酶）以及物理，化學(xué)和細(xì)菌劑。由于EGF會(huì)增加患癌癥的風(fēng)險(xiǎn)，因此抑制它會(huì)降低患癌癥的風(fēng)險(xiǎn)。