Description of DEAE-Dextran from Wuhan Fortuna Chemical Co.,Ltd

Specifications(COA) of DEAE-Dextran CAS 9015-73-0

Introduction of DEAE-Dextran is a cationic polymer derived from dextran (a glucose polysaccharide) modified with diethylaminoethyl (DEAE) groups, giving it a positive charge at neutral pH. Primarily used in molecular biology, it facilitates transfection by binding negatively charged DNA, forming complexes that adhere to cell membranes for uptake. It also serves as an anion exchanger in chromatography (e.g., DEAE-Sephadex) to purify proteins/nucleic acids. While largely replaced by lipofection or electroporation due to cytotoxicity at high doses, it remains cost-effective for specific cell types. Additional roles include nucleic acid precipitation and experimental drug/gene delivery. Optimal use requires pH control and minimized exposure to reduce cell damage.

Application of DEAE-Dextran is a chemically modified polysaccharide used primarily in molecular biology and biochemistry. Here's a structured overview:

Chemical Composition

Dextran Base: A polysaccharide composed of glucose units, known for its solubility and biocompatibility.

DEAE Modification: Diethylaminoethyl groups are attached, conferring a positive charge at physiological pH, making it a cationic polymer.

Key Applications

1.     Transfection

Mechanism: Binds negatively charged DNA via electrostatic interactions, forming complexes that adhere to cell membranes. This facilitates cellular uptake, potentially through endocytosis or membrane disruption.

Use Case: Historically employed for introducing DNA into mammalian cells (e.g., COS, HeLa). Efficiency varies by cell type and requires optimization to balance efficacy and cytotoxicity.

Current Status: Largely supplanted by lipofection and electroporation but remains cost-effective for specific applications.

2.     Chromatography

Ion Exchange Resin: DEAE-Dextran (e.g., DEAE-Sephadex) acts as a weak anion exchanger, separating proteins or nucleic acids based on charge. Used in purifying negatively charged biomolecules.

3.     Other Uses

Nucleic Acid Precipitation: Can aggregate DNA/RNA via charge neutralization.

Drug/Gene Delivery: Explored as a carrier for charged therapeutic molecules, though toxicity limits in vivo use.

Considerations

Cytotoxicity: High concentrations can damage cells, restricting exposure time in transfection protocols.

pH Sensitivity: Charge density depends on pH, requiring buffered conditions for optimal performance.

Conclusion

DEAE-Dextran is a versatile tool in biotechnology, leveraging its cationic properties for transfection and chromatography. While newer methods have reduced its prevalence, it remains valuable in niche applications where cost and simplicity are prioritized.

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Wuhan Fortuna Chemical Co.,Ltd is a professional manufacturer which provide DEAE-Dextran with good quality and best price, welcome to contact us to get COA/TDS/MSDS of DEAE-Dextran.