5-FAM NEW
| Price | $33 | $47 |
| Package | 50mg | 100mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-07-30 |
Product Details
| Product Name: 5-FAM | CAS No.: 76823-03-5 |
| Purity: 99.32% | Supply Ability: 10g |
| Release date: 2025/07/30 |
Product Introduction
Bioactivity
| Name | 5-FAM |
| Description | 5-FAM (5-Carboxyfluorescein) (5-Carboxyfluorescein) contains a carboxylic acid, which can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its excellent fluorescence quantum yield, relatively high absorptivity, and good water solubility, it also has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser. |
| Cell Research | Instructions 1. Preparation and storage a. Solubility: 5-FAM is usually provided in powder form and is easily soluble in DMSO, DMF, or aqueous solution with pH ≥ 8. b. Recommended stock solution concentration: 10–100 mM (adjusted according to experimental needs). Before use, it is recommended to prepare with anhydrous solvent and dispense in small volumes to avoid repeated freezing and thawing. 2. Biomolecule labeling (such as proteins, nucleic acids) a. Activation reaction: 5-FAM is usually combined with an activation reagent (such as EDC/NHS) to generate active esters to facilitate reaction with the amine group of the target molecule. b. Amine-free neutral buffer (such as MES or PBS, pH 6–8) is recommended for the reaction buffer. c. Binding step: Mix the active ester with the target molecule and react at room temperature for 1–2 hours. Optimize the pH and reaction time if necessary. d. Purification: Use dialysis or gel filtration to remove unreacted 5-FAM. 3. Fluorescence imaging a. Sample preparation: dilute 5-FAM to working concentration (usually 1–10 μM). b. Directly apply or incubate cells or tissue for 10–30 minutes, followed by washing with PBS to remove unbound dye. c. Microscope observation: Excitation wavelength: ~495 nm. Emission wavelength: ~520 nm (green fluorescence). |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 30 mg/mL (79.72 mM), Sonication is recommended. |
| Keywords | Inhibitor | inhibit | 5-FAM | 5FAM | 5 FAM |
| Related Compound Libraries | Human Metabolite Library |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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