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ChemicalBook >> CAS DataBase List >>HEPPS

HEPPS

CAS No.
16052-06-5
Chemical Name:
HEPPS
Synonyms
HEPE;EPPS;aEPPS;HEPPS;EPPS,99%;HEPPS(EPPS);HEPPS Buffer;EPPS USP/EP/BP;EPPS SIGMAULTRA;HEPPS ULTRAPURE
CBNumber:
CB9310937
Molecular Formula:
C9H20N2O4S
Molecular Weight:
252.33
MDL Number:
MFCD00006160
MOL File:
16052-06-5.mol
MSDS File:
SDS
Last updated:2025-01-27 09:38:02

HEPPS Properties

Melting point 237-239 °C(lit.)
Density 1.2684 (rough estimate)
refractive index 1.6800 (estimate)
storage temp. Sealed in dry,Room Temperature
solubility H2O: 1 M at 20 °C, clear, colorless
form Powder/Solid
color White
PH 5.0-6.5 (25℃, 0.1M in H2O)
pka 8.0(at 25℃)
PH Range 7.3 - 8.7
Water Solubility Soluble in water. 25.2 g/L (20°C)
λmax λ: 260 nm Amax: 0.055
λ: 280 nm Amax: 0.045
BRN 3957385
CAS DataBase Reference 16052-06-5(CAS DataBase Reference)
EWG's Food Scores 1
FDA UNII 8JN5PQW99C
EPA Substance Registry System 1-Piperazinepropanesulfonic acid, 4-(2-hydroxyethyl)- (16052-06-5)
Absorption ≤0.1 at 260 in H2O at 1M
≤0.1 at 280 in H2O at 1M
UNSPSC Code 12161700
NACRES NA.25

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS07
Signal word  Warning
Hazard statements  H302-H315-H319-H332-H335
Precautionary statements  P280-P305+P351+P338-P310
Hazard Codes  Xi
Risk Statements  36/37/38-22
Safety Statements  24/25-36-26
WGK Germany  3
RTECS  TM2649130
TSCA  Yes
HazardClass  IRRITANT
HS Code  29335995
Toxicity quail,LD50,oral,> 316mg/kg (316mg/kg),Ecotoxicology and Environmental Safety. Vol. 6, Pg. 149, 1982.
NFPA 704
1
2 1

HEPPS price More Price(43)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich E0276 EPPS BioPerformance Certified, cell culture tested, ≥99.5% (titration) 16052-06-5 25g $57.5 2023-06-20 Buy
TCI Chemical H0576 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic Acid [Good's buffer component for biological research] >98.0%(T) 16052-06-5 25g $108 2024-03-01 Buy
Alfa Aesar A13714 EPPS, 99% 16052-06-5 25g $31.65 2024-03-01 Buy
Alfa Aesar A13714 EPPS, 99% 16052-06-5 100g $100 2024-03-01 Buy
Sigma-Aldrich E9502 EPPS ≥99.5% (titration) 16052-06-5 10g $28.08 2024-03-01 Buy
Product number Packaging Price Buy
E0276 25g $57.5 Buy
H0576 25g $108 Buy
A13714 25g $31.65 Buy
A13714 100g $100 Buy
E9502 10g $28.08 Buy

HEPPS Chemical Properties,Uses,Production

Chemical Properties

white crystalline powder

Uses

HEPPS (EPPS) is a commonly used Good's buffer. It is often used as separator in ultrathin isoelectric focusing gels and enhances the resolution of phosphoglucomutase. It is used as a buffering agent in biology and biochemistry. HEPPS is a biological buffer with a useful pH range of 7.3-8.7 and a pKa=8.0 at 25°C. Research on mice with Alzheimer's disease-like amyloid beta plaques has shown that HEPPS can cause the plaques to break up, reversing some of the symptoms in the mice. HEPPS was reported to dissociate amyloid beta oligomers in patients' plasma samples enabling blood diagnosis of Alzheimer's disease.

Definition

ChEBI: HEPPS is a N-(sulfoalkyl)piperazine and a N-(2-hydroxyethyl)piperazine.

General Description

HEPPS, or N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), is a zwitterionic buffering agent commonly used in biochemical and molecular biology research. Its mechanism of action is rooted in its ability to maintain a stable pH environment, which is crucial for various enzymatic reactions and protein studies. HEPPS is especially favoured for its buffering capacity in the physiological pH range, typically between pH 6.8 and 8.2, making it ideal for biological assays and cell culture experiments. This compound is extensively utilized in preparing cell culture media, where maintaining a consistent pH is essential for cell viability and growth. Additionally, HEPPS is employed in biochemical assays, such as enzyme kinetics studies, where precise pH control is necessary to accurately measure enzymatic activity. Furthermore, HEPPS has found applications in molecular biology techniques, including nucleic acid purification, PCR (polymerase chain reaction), and protein analysis by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Its compatibility with various biological systems and stability under physiological conditions make HEPPS a valuable tool in research laboratories for maintaining optimal pH conditions and ensuring reliable experimental outcomes in various biochemical and molecular biology studies.

Flammability and Explosibility

Not classified

HEPPS Preparation Products And Raw materials

Raw materials

Preparation Products

Global( 371)Suppliers
Supplier Tel Email Country ProdList Advantage
Hubei Yiling biotechnology Co Ltd
+86-18163349544 +86-18163349544 mollylucking@foxmail.com China 2767 58
Suzhou Genemill Biotechnology Co.,Ltd
+8615862502491 aimeegao@genemill.com China 39 58
Hebei Mujin Biotechnology Co.,Ltd
+86 13288715578 +8613288715578 sales@hbmojin.com China 12823 58
Hebei Chuanghai Biotechnology Co., Ltd
+8615531151365 mina@chuanghaibio.com China 18140 58
Henan Bao Enluo International TradeCo.,LTD
+86-17331933971 +86-17331933971 deasea125996@gmail.com China 2472 58
hebei hongtan Biotechnology Co., Ltd
+852-6619 3215 +86-17731935328 sales03@chemcn.cn China 970 58
Hebei Zhuanglai Chemical Trading Co.,Ltd
+8613343047651 admin@zlchemi.com China 3692 58
Shanghai Daken Advanced Materials Co.,Ltd
+86-371-+86-371-66670886 info@dakenam.com China 19840 58
Henan Tianfu Chemical Co.,Ltd.
+86-0371-55170693 +86-19937530512 info@tianfuchem.com China 21631 55
Shanghai Time Chemicals CO., Ltd.
+86-021-57951555 +8617317452075 jack.li@time-chemicals.com China 1803 55

View Lastest Price from HEPPS manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
HEPPS pictures 2025-03-17 HEPPS
16052-06-5
US $32.00 / kg 1kg 99% 5000kg/week Hebei Zhuanglai Chemical Trading Co.,Ltd
EPPS pictures 2025-03-17 EPPS
16052-06-5
US $0.00 / KG 1KG 99% 50000KG/month Hebei Mujin Biotechnology Co.,Ltd
HEPPS pictures 2025-03-13 HEPPS
16052-06-5
US $0.00 / KG 1KG 99% 3500kg/month WUHAN FORTUNA CHEMICAL CO., LTD
  • HEPPS pictures
  • HEPPS
    16052-06-5
  • US $32.00 / kg
  • 99%
  • Hebei Zhuanglai Chemical Trading Co.,Ltd
  • EPPS pictures
  • EPPS
    16052-06-5
  • US $0.00 / KG
  • 99%
  • Hebei Mujin Biotechnology Co.,Ltd
  • HEPPS pictures
  • HEPPS
    16052-06-5
  • US $0.00 / KG
  • 99%
  • WUHAN FORTUNA CHEMICAL CO., LTD
HEPPS(EPPS) 4-(2-Hydroxyethyl)pi HEPE 3-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]PROPANESULFONIC ACID 3-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]PROPANESULFONIC ACID N-(2-HYDROXYETHYL)-N'-(3-SULFOPROPYL)-PIPERAZINE N-[2-HYDROXYETHYL]PIPERAZINE-N'-[3-PROPANESULFONIC ACID] N-2-HYDROXYETHYLPIPERAZINE-N'-3-PROPANESULPHONIC ACID aEPPS HEPPS N-(2-Hydroxyethyl)piperazine-N-3-propanesulfonic acid HEPPS, ULTROL Grade HEPPS~4-(2-Hydroxyethyl)-1-piperazinepropanesulphonic acid EPPS FREE ACID, BIOTECHNOLOGY PERFORMANC E CERTIFIED EPPS, 99% (4-(2-HYDROXYETHYL)-1-PIPERAZI NE -PROPANESULFONIC ACID) EPPS SIGMAULTRA HEPPS CELL CULTURE REAGENT CRYSTALLINE EPPS 99% (4-(2-HYDROXYETHYL)-1-PIPERAZINE -PROPANESULFONIC ACID) 99+% ULTRA PURE BUFFER GRADE EPPS, for biochemistry, 99% Hydroxyethyl)piperazine-N'-3-propane-sulfonic acid 1-Piperazinepropanesulfonic acid, 4-(2-hydroxyethyl)- 4-(2-hydroxyethyl)-1-piperazinepropanesulphonic acid N-(hydroxyethyl)piperazine-N’-propanesulfonic acid (HEPPS) 4-(2-HYDROXYETHYL)-1-PIPERAZINEPROPANESULFONIC ACID EPPS HEPPS ULTRAPURE HEPPS (4-(2-HYDROXYETHYL)PIPERAZINE-1-PROPANESULFONIC ACID) 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic Acid  HEPPS/EPPS/HEPE 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid[HEPPS] HEPPS 3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic Acid 4-Hydroxyerhylpiperazine-1-propanesulfonic acid EPPS,99% EPPS Vetec(TM) reagent grade, 99% HEPPS/ 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid 4-(2-hydroxyethyl)-1-piperazinepropanesulfonicaci EPPS HEPPS LABOTEST-BB LT00138110 4-(2-HYDROXYETHYL)-1-PIPERAZINEPROPANESULFONIC ACID 4-(2-HYDROXYETHYL)PIPERAZINE-1-PROPANESULFONIC ACID N-(2-Hydroxyethyl)piperazine-N'-3-propanesulphonic acid >99% 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic Acid [Good's buffer component for biological research] Epps, 99%, for biochemistry HeppsN-(2-Hydroxyethyl)Piperazine-NPropanesulfonicAcid Epps(N-[2-Hydroxyethyl]Piperazine-N3-PropaneSulfonicAcid]) 3-[4-(2-Hydroxyethyl)-1-piperazinyl]-1-propanesulfonic acid 3-[4-(2-Hydroxyethyl)piperazine-1-yl]-1-propanesulfonic acid 3-[4-(2-Hydroxyethyl)piperazino]-1-propanesulfonic acid 4-(2-Hydroxyethyl)-1-piperazinepropane-1-sulfonic acid HEPPS Buffer 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid, 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid, HEPPS, N-(2-Hydroxyethyl)piperazine-Nμ-(3-propanesulfonic acid) 3-[4-(2-Hydroxyethyl)piperazin-1-yl]propane-1-sulfonic acid [2-hydroxyethyl]piperazine-n’-[3-propancesulfonicacid] N-(Hydroxyethyl)piperazine-N'-propane sulfonic acid 3-(4-(2-Hydroxyethyl)piperazin-1-yl)propane-1-sulfonic N-(2-Hydroxyethyl)piperazine-N'-(propanesulphonic acid) HEPPS, ≥99.0%(T) HEPPS, ≥99.5%(T) 4-(2-HYDROXYETHYL)PIPERAZINE-1-PROPANESULFONIC ACID;EPPS