HEPPS
- CAS No.
- 16052-06-5
- Chemical Name:
- HEPPS
- Synonyms
- HEPE;EPPS;aEPPS;HEPPS;EPPS,99%;HEPPS(EPPS);HEPPS Buffer;EPPS USP/EP/BP;EPPS SIGMAULTRA;HEPPS ULTRAPURE
- CBNumber:
- CB9310937
- Molecular Formula:
- C9H20N2O4S
- Molecular Weight:
- 252.33
- MDL Number:
- MFCD00006160
- MOL File:
- 16052-06-5.mol
- MSDS File:
- SDS
| Melting point | 237-239 °C(lit.) |
|---|---|
| Density | 1.2684 (rough estimate) |
| refractive index | 1.6800 (estimate) |
| storage temp. | Sealed in dry,Room Temperature |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| form | Powder/Solid |
| color | White |
| PH | 5.0-6.5 (25℃, 0.1M in H2O) |
| pka | 8.0(at 25℃) |
| PH Range | 7.3 - 8.7 |
| Water Solubility | Soluble in water. 25.2 g/L (20°C) |
| λmax |
λ: 260 nm Amax: 0.055 λ: 280 nm Amax: 0.045 |
| BRN | 3957385 |
| CAS DataBase Reference | 16052-06-5(CAS DataBase Reference) |
| EWG's Food Scores | 1 |
| FDA UNII | 8JN5PQW99C |
| EPA Substance Registry System | 1-Piperazinepropanesulfonic acid, 4-(2-hydroxyethyl)- (16052-06-5) |
| Absorption |
≤0.1 at 260 in H2O at 1M ≤0.1 at 280 in H2O at 1M |
| UNSPSC Code | 12161700 |
| NACRES | NA.25 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H302-H315-H319-H332-H335 | |||||||||
| Precautionary statements | P280-P305+P351+P338-P310 | |||||||||
| Hazard Codes | Xi | |||||||||
| Risk Statements | 36/37/38-22 | |||||||||
| Safety Statements | 24/25-36-26 | |||||||||
| WGK Germany | 3 | |||||||||
| RTECS | TM2649130 | |||||||||
| TSCA | Yes | |||||||||
| HazardClass | IRRITANT | |||||||||
| HS Code | 29335995 | |||||||||
| Toxicity | quail,LD50,oral,> 316mg/kg (316mg/kg),Ecotoxicology and Environmental Safety. Vol. 6, Pg. 149, 1982. | |||||||||
| NFPA 704 |
|
HEPPS price More Price(43)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | E0276 | EPPS BioPerformance Certified, cell culture tested, ≥99.5% (titration) | 16052-06-5 | 25g | $57.5 | 2023-06-20 | Buy |
| TCI Chemical | H0576 | 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic Acid [Good's buffer component for biological research] >98.0%(T) | 16052-06-5 | 25g | $108 | 2024-03-01 | Buy |
| Alfa Aesar | A13714 | EPPS, 99% | 16052-06-5 | 25g | $31.65 | 2024-03-01 | Buy |
| Alfa Aesar | A13714 | EPPS, 99% | 16052-06-5 | 100g | $100 | 2024-03-01 | Buy |
| Sigma-Aldrich | E9502 | EPPS ≥99.5% (titration) | 16052-06-5 | 10g | $28.08 | 2024-03-01 | Buy |
HEPPS Chemical Properties,Uses,Production
Chemical Properties
white crystalline powder
Uses
HEPPS (EPPS) is a commonly used Good's buffer. It is often used as separator in ultrathin isoelectric focusing gels and enhances the resolution of phosphoglucomutase. It is used as a buffering agent in biology and biochemistry. HEPPS is a biological buffer with a useful pH range of 7.3-8.7 and a pKa=8.0 at 25°C. Research on mice with Alzheimer's disease-like amyloid beta plaques has shown that HEPPS can cause the plaques to break up, reversing some of the symptoms in the mice. HEPPS was reported to dissociate amyloid beta oligomers in patients' plasma samples enabling blood diagnosis of Alzheimer's disease.
Definition
ChEBI: HEPPS is a N-(sulfoalkyl)piperazine and a N-(2-hydroxyethyl)piperazine.
General Description
HEPPS, or N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), is a zwitterionic buffering agent commonly used in biochemical and molecular biology research. Its mechanism of action is rooted in its ability to maintain a stable pH environment, which is crucial for various enzymatic reactions and protein studies. HEPPS is especially favoured for its buffering capacity in the physiological pH range, typically between pH 6.8 and 8.2, making it ideal for biological assays and cell culture experiments. This compound is extensively utilized in preparing cell culture media, where maintaining a consistent pH is essential for cell viability and growth. Additionally, HEPPS is employed in biochemical assays, such as enzyme kinetics studies, where precise pH control is necessary to accurately measure enzymatic activity. Furthermore, HEPPS has found applications in molecular biology techniques, including nucleic acid purification, PCR (polymerase chain reaction), and protein analysis by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Its compatibility with various biological systems and stability under physiological conditions make HEPPS a valuable tool in research laboratories for maintaining optimal pH conditions and ensuring reliable experimental outcomes in various biochemical and molecular biology studies.
Flammability and Explosibility
Not classified
HEPPS Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Hubei Yiling biotechnology Co Ltd | +86-18163349544 +86-18163349544 | mollylucking@foxmail.com | China | 2767 | 58 |
| Suzhou Genemill Biotechnology Co.,Ltd | +8615862502491 | aimeegao@genemill.com | China | 39 | 58 |
| Hebei Mujin Biotechnology Co.,Ltd | +86 13288715578 +8613288715578 | sales@hbmojin.com | China | 12823 | 58 |
| Hebei Chuanghai Biotechnology Co., Ltd | +8615531151365 | mina@chuanghaibio.com | China | 18140 | 58 |
| Henan Bao Enluo International TradeCo.，LTD | +86-17331933971 +86-17331933971 | deasea125996@gmail.com | China | 2472 | 58 |
| hebei hongtan Biotechnology Co., Ltd | +852-6619 3215 +86-17731935328 | sales03@chemcn.cn | China | 970 | 58 |
| Hebei Zhuanglai Chemical Trading Co.,Ltd | +8613343047651 | admin@zlchemi.com | China | 3692 | 58 |
| Shanghai Daken Advanced Materials Co.,Ltd | +86-371-+86-371-66670886 | info@dakenam.com | China | 19840 | 58 |
| Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 | info@tianfuchem.com | China | 21631 | 55 |
| Shanghai Time Chemicals CO., Ltd. | +86-021-57951555 +8617317452075 | jack.li@time-chemicals.com | China | 1803 | 55 |
View Lastest Price from HEPPS manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2025-03-17 | HEPPS
16052-06-5
|
US $32.00 / kg | 1kg | 99% | 5000kg/week | Hebei Zhuanglai Chemical Trading Co.,Ltd | |
 |
2025-03-17 | EPPS
16052-06-5
|
US $0.00 / KG | 1KG | 99% | 50000KG/month | Hebei Mujin Biotechnology Co.,Ltd | |
 |
2025-03-13 | HEPPS
16052-06-5
|
US $0.00 / KG | 1KG | 99% | 3500kg/month | WUHAN FORTUNA CHEMICAL CO., LTD |