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ChemicalBook >> CAS DataBase List >>EC 3.4.24.4

EC 3.4.24.4

CAS No.
9036-06-0
Chemical Name:
EC 3.4.24.4
Synonyms
PROTEASE;PRONASE;PRONASE E;protease from streptomyces griseus;Proteinase E;Proteline;Pronase-p;Protenaza1;ACTINASE E;PRONASE(R)
CBNumber:
CB7670039
Molecular Formula:
NULL
Molecular Weight:
0
MDL Number:
MFCD02253017
MOL File:
Mol file
MSDS File:
SDS
Last updated:2025-04-17 18:22:24

EC 3.4.24.4 Properties

storage temp. 2-8°C
solubility H2O: 5-20 mg/mL
form powder
color slightly brown
biological source Streptomyces griseus
Water Solubility water: 10mg/mL
Specific Activity ≥70,000proteolytic units/g dry wt
UNSPSC Code 12352207
NACRES NA.75

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictogramsGHS hazard pictograms
GHS07,GHS08
Signal word  Danger
Hazard statements  H315-H319-H334-H335
Precautionary statements  P302+P352-P305+P351+P338
Hazard Codes  Xn
Risk Statements  36/37/38-42
Safety Statements  22-24-26-36/37-24/25
WGK Germany  1
RTECS  UK9540000
F  10
HS Code  2934999090

EC 3.4.24.4 price More Price(24)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich P0652 Protease from Streptomyces sp. Type XXI, ≥15?units/mg solid 9036-06-0 500units $688 2024-03-01 Buy
Sigma-Aldrich 7433 Pronase E 9036-06-0 1g $390 2024-03-01 Buy
Sigma-Aldrich 53702 Protease from Streptomyces griseus 9036-06-0 10ku $74.6 2024-03-01 Buy
Sigma-Aldrich 53702 Protease from Streptomyces griseus 9036-06-0 25ku $89.5 2024-03-01 Buy
Sigma-Aldrich P8811 Protease from Streptomyces griseus powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid 9036-06-0 100mg $59.7 2024-03-01 Buy
Product number Packaging Price Buy
P0652 500units $688 Buy
7433 1g $390 Buy
53702 10ku $74.6 Buy
53702 25ku $89.5 Buy
P8811 100mg $59.7 Buy

EC 3.4.24.4 Chemical Properties,Uses,Production

Uses

Pronase E can be used to degrade antheraea pernyi silk fibroin films.

General Description

Note: 1 KU = 1000 units.

Biochem/physiol Actions

This product is a mixture of at least three caseinolytic activities and one aminopeptidase activity. The caseinolytic enzymes were named as Streptomyces griseus Protease A, Streptomyces griseus Protease B and Streptomyces griseus Trypsin. This product may be used when extensive or complete degradation of protein is required. This protease mixture is highly nonspecific and can digest casein to the extent of >70% as mono-amino acids.

in vivo

Experimental Procedure for Isolating Primary Hematopoietic Stem Cells (HSCs) from Mice Using Pronase E[2]1. Animal Anesthesia and Abdominal Exposure: (1) Anesthetize male or female mice. (2) Under sterile conditions, expose the liver via abdominal incision. 2. Liver Perfusion Procedure: (1) Perform liver perfusion through the portal vein using Hanks' Balanced Salt Solution (HBSS) to remove blood and impurities. (2) Conduct enzymatic perfusion with Pronase E and collagenase to dissociate liver tissue. 3. Liver Extraction and Cell Release: (1) Carefully extract the liver from the abdominal cavity using sterile forceps, and quickly transfer it to a sterile culture dish. (2) Gently peel the liver capsule and apply mild mechanical agitation to release cells from the liver tissue. 4. Cell Digestion and Preliminary Centrifugation: (1) Suspend the released cells in a mixed enzyme solution containing Pronase E, collagenase, and DNase, and digest at 37°C for 20 minutes. (2) Perform two rounds of low-speed centrifugation (50 g, 3 minutes) to separate liver cells, and collect the cell suspension. 5. HSC Separation and Density Gradient Centrifugation: (1) Further centrifuge the cell suspension (450 g, 8 minutes) and collect the pellet. (2) Resuspend the pellet in 18% Nycodenz solution as the basis for density gradient separation. (3) Construct a density gradient with 12% Nycodenz, 8.2% Nycodenz, and Gey's Balanced Salt Solution (GBSS). (4) Isolate and purify HSCs from the 8.2% Nycodenz layer through density gradient centrifugation. Note: This protocol provides standard operating procedures; please adjust and optimize according to specific experimental needs and conditions.

EC 3.4.24.4 Preparation Products And Raw materials

Raw materials

Preparation Products

Global( 136)Suppliers
Supplier Tel Email Country ProdList Advantage
Henan Tianfu Chemical Co.,Ltd. 		+86-0371-55170693 +86-19937530512 info@tianfuchem.com China 21629 55
career henan chemical co 		+86-0371-86658258 +8613203830695 sales@coreychem.com China 29860 58
Chongqing Chemdad Co., Ltd 		+86-023-6139-8061 +86-86-13650506873 sales@chemdad.com China 39894 58
Zhengzhou Alfa Chemical Co.,Ltd 		+8618530059196 sale04@alfachem.cn China 12132 58
Creative Enzymes 		1-516-855-7709 info@creative-enzymes.com United States 8748 58
TargetMol Chemicals Inc. 		+1-781-999-5354 +1-00000000000 marketing@targetmol.com United States 32159 58
Hubei Ipure Biology Co., Ltd 		+8613367258412 ada@ipurechemical.com China 10244 58
HONG KONG IPURE BIOLOGY CO.,LIMITED 		86 18062405514 18062405514 ada@ipurechemical.com CHINA 3461 58
ANHUI WITOP BIOTECH CO., LTD 		+8615255079626 eric@witopchemical.com China 23541 58
Wuhan Fortuna Chemical Co., Ltd 		+86-027-59207850 info@fortunachem.com China 5969 58

View Lastest Price from EC 3.4.24.4 manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Pronase E pictures 2025-04-16 Pronase E
9036-06-0
 US $0.00-0.00 / g 1g 98.0% 1kg/month WUHAN FORTUNA CHEMICAL CO., LTD
Pronase E (Activity ≥ 7000 U/g) pictures 2024-11-19 Pronase E (Activity ≥ 7000 U/g)
9036-06-0
 US $43.00-32.00 / mg 10g TargetMol Chemicals Inc.
Pronase E (Activity ≥ 7000 U/g) pictures 2024-11-19 Pronase E (Activity ≥ 7000 U/g)
9036-06-0
 US $43.00-32.00 / mg 10g TargetMol Chemicals Inc.
  • Pronase E pictures
  • Pronase E
    9036-06-0
  • US $0.00-0.00 / g
  • 98.0%
  • WUHAN FORTUNA CHEMICAL CO., LTD
s.Griseusproteinase Streptomycesgriseusprotease Streptomycesgriseusproteinase ACTINASE E, STREPTOMYCES GRISEUS Protease from Streptomyces griseus,Actinase E, Pronase E pronasef.streptomycesgriseus Proteinase,streptomycesgriseus Proteline Protenaza1 s.Griseusprotease Pronase E froM StreptoMyces griseus ca. 6-8 DMC-U/Mg lyophil. Empynase PD Pronase-p EC 3.4.24.4 EC 3.4.24.31 EC 3.4.24.32 GRISEUS PROTEASE PRONASE F. STREPTOMYCES GRISEUS LYOPH., POWDER, 6 U/MG PROTEASE TYPE XIV BACTERIAL FROM*STREPTO MYCES GRISE PronaseEExStreptomycesGriseus Pronase E, Actinase E Pronase from Streptomyces griseus [EC 3.4.24.4] from Streptomyces griseus Actinase E, Pronase E Type XIV protease ACTINASE E pronase E from streptomyces griseus PRONASE Protease, Streptomycesgriseus, Nuclease-Free protease bacterial (pronase E)*aseptically filled protease type xiv bacterial from*streptomyces gri protease type xxi from streptomyces*griseus PROTEASE TYPE IX PROTEASE (STREPTOMYCES GRISEUS) PROTEASE TYPE XIV PROTEASE TYPE XXI PRONASE(R) PRONASE(R) PROTEASE 5-[(3Z)-4,8-Dimethyl-3,7-nonadien-1-yl]octahydro-2H-1,7-dioxacyclopenta[cd]indene-2,6-diyl diacetate Protease from Streptomyces sp. PronaseE, 7000U/g Native Streptomyces griseus Protease Pronase E (Activity ≥ 4000 U/mg) Pronase E (Activity ≥ 7000 U/g) Protease ex. Aspergillus Oryzae (Fungal) (Type 1 - Acidic, powder), 500kHUT/g Protease ex. Bacillus Licheniformis (Type 4 - Neutral, powder), 10U/mg Protease ex. Bacillus Sp. (Type 5 - Neutral, granules), 8kNPU/g Protease ex. Bacillus Subtilis (Type 2 - Alkaline, granules), 100kAPU/g Protease ex. Bacillus Subtilis (Type 3 - Alkaline, granules), 1000kAPU/g Protease ex. Bacillus Subtilis (Type 6 - Neutral, powder), 100kNPU/g ~4 units/mg solid， powder Protease from Streptomyces griseus Type XIV protease from streptomyces griseus PROTEASE PRONASE E PRONASE Proteinase E Pronase Protease 3.5units/mg Pronase, from Streptomyces griseus