| Identification | Back Directory | [Name]
6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein | [CAS]
82855-40-1 | [Synonyms]
JOE816
2',7'-diOMe)
6-Fam(4‘,5’-diCl
4',5'-Dichloro-3',6'-dihydroxy-2',7'-dimethoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylic acid | [Molecular Formula]
C23H14Cl2O9 | [MOL File]
82855-40-1.mol | [Molecular Weight]
505.26 |
| Chemical Properties | Back Directory | [Boiling point ]
780.5±60.0 °C(Predicted) | [density ]
1.79±0.1 g/cm3(Predicted) | [form ]
Solid | [pka]
3.47±0.20(Predicted) | [color ]
Brown to reddish brown |
| Hazard Information | Back Directory | [Uses]
JOE is a fluorescent dye and can be used as a fluorescent label in DNA sequencing, PCR, and LCR amplifications. JOE possesses an absorbance of 525 nm and an emission of 550 nm[1]. | [Definition]
ChEBI: 6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein is a fluorescein compound having chloro substituents in the 4'- and 5'-positions, methoxy substituents in the 2'- and 7'-positions and a carboxy substituent at the 6-position. It has a role as a fluorochrome. It is functionally related to a fluorescein (lactone form). | [References]
[1] Tsybulsky DA, et al. 4',5'-Dichloro-2',7'-dimethoxy-5(6)-carboxyfluorescein (JOE): synthesis and spectral properties of oligonucleotide conjugates. J Org Chem. 2012 Jan 20;77(2):977-84. DOI:10.1021/jo202229t |
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