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ChemicalBook--->CAS DataBase List--->2635-84-9

2635-84-9

2635-84-9 Structure

2635-84-9 Structure
IdentificationBack Directory
[Name]

P-NITROPHENYL BUTYRATE
[CAS]

2635-84-9
[Synonyms]

4-NITROPHENYL BUTYRATE
P-NITROPHENYL BUTYRATE
4-Nitrophenol butyrate
PARA-NITROPHENYLBUTYRATE
(4-nitrophenyl) butanoate
4-Nitrophenyl butyrate >=98%
BUTYRIC ACID 4-NITROPHENYL ESTER
Butanoic acid p-nitrophenyl ester
Butanoic acid, 4-nitrophenyl ester
p-Nitrophenyl butyrate, Butyric acid 4-nitrophenyl ester
4-Nitrophenyl butyrate,p-Nitrophenyl butyrate, Butyric acid 4-nitrophenyl ester
[EINECS(EC#)]

258-258-3
[Molecular Formula]

C10H11NO4
[MDL Number]

MFCD00047734
[MOL File]

2635-84-9.mol
[Molecular Weight]

209.2
Chemical PropertiesBack Directory
[Boiling point ]

184-185 °C
[density ]

1.19 g/mL at 20 °C(lit.)
[storage temp. ]

-20°C
[solubility ]

chloroform: 0.1 g/mL, clear, faintly yellow
[form ]

A neat oil
[color ]

Colorless to light yellow
[BRN ]

1879729
[InChIKey]

DVDUMIQZEUTAGK-UHFFFAOYSA-N
Safety DataBack Directory
[Hazard Codes ]

Xi
[Risk Statements ]

43
[Safety Statements ]

36/37
[WGK Germany ]

3
[HS Code ]

29156000
Hazard InformationBack Directory
[Description]

P-nitrophenyl butyrate also kown as 4-nitrophenyl butyrate, is a probe for lipase activity. It is hydrolyzed by lipase in the water ambient condition, giving rise to p-nitrophenol and butyric acid. P-nitrophenol actually occurs in the anionic form, p-nitrophenolate, giving a yellowish coloration and absorbing light at 410 nm in an alkaline buffer such as Tris-HCl pH 7.5.
[Uses]

4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.
[Uses]

A probe for lipase activity.
[Definition]

ChEBI: A butyrate ester resulting from the formal condensation of the hydroxy group of 4-nitrophenol with the carboxy group of butyric acid.
[General Description]

4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.
[References]

[1] POHANKA M. Biosensors and Bioassays Based on Lipases, Principles and Applications, a Review.[J]. Molecules, 2019. DOI:10.3390/molecules24030616.
[2] WENTING SHANG. Enzymatic hydrolysis of p-nitrophenyl butyrate in CO2-based micelle systems[J]. Journal of chemical technology and biotechnology, 2013, 89 9: 1324-1329. DOI:10.1002/jctb.4246.
[3] SIGRUN H. STERRI Frode F Bj?rn A Johnsen. A radiochemical assay method for carboxylesterase, and comparison of enzyme activity towards the substrates methyl [1-14C] butyrate and 4-nitrophenyl butyrate[J]. Biochemical pharmacology, 1985, 34 15: Pages 2779-2785. DOI:10.1016/0006-2952(85)90579-9.
[4] BISHNU PRASAD PANDEY. Bergenia pacumbis from Nepal, an astonishing enzymes inhibitor.[J]. BMC Complementary Medicine and Therapies, 2020: 198. DOI:10.1186/s12906-020-02989-2.
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