| Identification | Back Directory | [Name]
sodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate | [CAS]
143556-24-5 | [Synonyms]
L 012 sodium salt
1H-Benz[e]indole-6,8-disulfonic acid, 1,1,2-trimethyl-
sodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate
8-Amino-5-chloro-2,3-dihydro-7-phenyl-pyrido[3,4-d]pyridazine sodium salt | [Molecular Formula]
C13H8ClN4NaO2 | [MDL Number]
MFCD27991293 | [MOL File]
143556-24-5.mol | [Molecular Weight]
310.671 |
| Chemical Properties | Back Directory | [storage temp. ]
-20°C | [solubility ]
Soluble to 20 mM in water and to 100 mM in DMSO | [form ]
Solid | [color ]
White to yellow | [Water Solubility ]
H2O: 2mg/mL, clear |
| Hazard Information | Back Directory | [Uses]
L 012 Sodium Salt is a chemiluminescent dye used to detect superoxide in biological systems, specifically in whole blood, inflammatory cells, and vascular tissue. | [Biological Activity]
L-012 is a luminol analog and a widely used reactive oxygen and nitrogen species (RONS; ROS & RNS) chemiluminescence (CL) probe both in cultures and in animals in vivo. L-012 displays significantly higher CL yield and sensitivity than luminollucigenin and MCLA. Instead of reacting with superoxide anion (O2-) directlyL-012 (LumH2) is converted via a one-electron oxidation (catalyzed by peroxidase in the presence of H2O2) to the LumH· radical formwhich then reacts with oxygen (O2) to yield O2- and L-012 quinone (q-Lum). The O2- in turn reacts with the LumH· radicalleading eventually to an endoperoxide th at decomposes to emit luminescence. To a less extendH2O2 can also react with q-Lum to yield luminescence. Although not specific to NADPH oxidase-derived RONSL-012 is also commonly used for screening NADPH oxidase inhibitors. | [in vivo]
L-012 sodium salt is well distributed in the mouse body and mediates a strong ROS/RNS-dependent luminescent signal in vivo and is useful for monitoring the development and regulation of inflammation in living organisms[2]. | [storage]
Store at -20°C |
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